Phospho-SHIP1 (Tyr1020) Antibody

• 产品编号：CST-3941S      品牌：CST       原厂货号：3941S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-SHIP1 (Tyr1021) Antibody只能检测内源的在tyr1020位点磷酸化的鼠SHIP1蛋白(或在Tyr1021位点磷酸化的人SHIP1蛋白)。多克隆抗体是通过合成鼠源对应的SHIP Tyr1020位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。包含SH2的肌糖磷酸酶1(SHIP1)是造血组织中的磷酸酶，水解磷脂酰肌醇-3,4,5-三磷酸盐成磷脂酰肌醇-3,4-二磷酸盐(1)。SHIP1是细胞质磷酸酶在其N-末端有SH2区域 和C-末端的两个NPXY Shc结合区域(1,2)。受体交叉结合后, SHIP首先被招募到膜结合处，通过其SH2区域结合到ITIM域的磷酸化酪氨酸上(2),这在酪氨酸磷酸化NPXY域之后(2)。细胞膜的重新定位和NPXY域的磷酸化对SHIP1的调控功能非常重要(3-5)。它通过PI3K和Akt通路影响钙流，细胞的存活，生长，细胞周期和凋亡(3-5)。Tyr1021定位于SHIP1的一个NPXY域内, 而且其磷酸化对SHIP1功能是非常重要的(6)。

1. Tridandapani, S. et al. (1997) Mol Cell Biol 17, 4305-11.
2. Liu, L. et al. (1997) J Biol Chem 272, 8983-8.
3. Malbec, O. et al. (2001) J Biol Chem 276, 30381-91.
4. Carver, D.J. et al. (2000) Blood 96, 1449-56.
5. Scharenberg, A.M. et al. (1998) EMBO J 17, 1961-72.
6. Sattler, M. et al. (2001) J Biol Chem 276, 2451-8.

原厂资料：

Specificity / Sensitivity

Phospho-SHIP1 (Tyr1021) Antibody detects endogenous levels of SHIP1 only when phosphorylated at tyrosine 1020 of mouse SHIP1 (or Tyr1021 of human SHIP1).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr1020 of mouse SHIP. Antibodies are purified by protein A and peptide affinity chromatography.

Background

SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate (1). SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus (1,2). Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif (2), followed by tyrosine phosphorylation on the NPXY motif (2). The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1 (3-5). Its effect on calcium flux, cell survival, growth, cell cycle arrest and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1021 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6).

1. Tridandapani, S. et al. (1997) Mol Cell Biol 17, 4305-11.
2. Liu, L. et al. (1997) J Biol Chem 272, 8983-8.
3. Malbec, O. et al. (2001) J Biol Chem 276, 30381-91.
4. Carver, D.J. et al. (2000) Blood 96, 1449-56.
5. Scharenberg, A.M. et al. (1998) EMBO J 17, 1961-72.
6. Sattler, M. et al. (2001) J Biol Chem 276, 2451-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.