Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-3939S      品牌：CST       原厂货号：3939S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (Alexa Fluor® 488)只能分别检测内源的在tyr694和Tyr699位点磷酸化的 Stat5a和Stat5b 蛋白。此单克隆抗体是通过合成对应的Stat5a Tyr694位点周围的磷肽段来免疫动物而获得。此抗体在优化的条件下与Alexa Fluor® 488 偶联，F/P 比例为2-6。CST公司生产的此抗体与Alexa Fluor® 488荧光染料偶联，并通过人源细胞的流式细胞仪的测试。未偶联的抗体与偶联了荧光染料的抗体呈现出相同的同物种交叉反应。Stat5的激活是通过一系列的配体的结合而完成，包括 IL-2, GM-CSF,生长激素和泌乳刺激素。磷酸化Tyr694位点对 Stat5的活性是必须的(1,2)。此位点的磷酸化是在红细胞生成素的作用下由Src介导而完成(3)。 Stat5在某些白血病类型的细胞中有组成性的活性(4)。用IL-3处理的内皮细胞中有磷酸化的Stat5，这就表明它参与了血管再生和细胞的运动(5)。在不同细胞类型中 Stat5a 和 Stat5b 是被独立调控和激活的。例如,干扰素处理后在U-937细胞中Stat5a的活性较强但在HeLa细胞中Stat5b的活性较强(6)。

1. Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
2. Wakao, H. et al. (1994) EMBO J 13, 2182-91.
3. Okutani, Y. et al. (2001) Oncogene 20, 6643-50.
4. Demoulin, J.B. et al. (1999) J Biol Chem 274, 25855-61.
5. Dentelli, P. et al. (1999) J Immunol 163, 2151-9.
6. Meinke, A. et al. (1996) Mol Cell Biol 16, 6937-44.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey, Bovine

Specificity / Sensitivity

Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (Alexa Fluor® 488) detects endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr694 of Stat5a. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.

Description

This Cell Signaling Technology (CST) antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry in human cells. CST expects that Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (Alexa Fluor® 488) will display the same species cross-reactivity as the unconjugated antibody (Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb #9314).

Background

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

1. Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
2. Wakao, H. et al. (1994) EMBO J 13, 2182-91.
3. Okutani, Y. et al. (2001) Oncogene 20, 6643-50.
4. Demoulin, J.B. et al. (1999) J Biol Chem 274, 25855-61.
5. Dentelli, P. et al. (1999) J Immunol 163, 2151-9.
6. Meinke, A. et al. (1996) Mol Cell Biol 16, 6937-44.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide, 2 mg/ml BSA. Store at 4°C. Protect from light.
Do not freeze.