Rab3A Antibody

• 产品编号：CST-3930S      品牌：CST       原厂货号：3930S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rab3A Antibody识别内源性的Rab3A总蛋白。该多克隆抗体用与人类Rab3A蛋白中Glu391位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白亲和层析纯化而得。Rab家族的蛋白质包括小的、单体的GTP酶，对调节胞内囊泡转运至关重要。Rab3亚家族成员，包括Rab3A-3D，参与神经递质和激素的胞吐（1）。Rab3A主要表达在神经元（2）、神经内分泌细胞（如大鼠PC-12细胞）和人胰岛β细胞（3,4）。作为分子开关，在激活的GTP结合的Rab3A和失活的GDP结合的形式之间切换，Rab3a抑制晚期膜释放时的突触囊泡和嗜铬颗粒分泌（5,6）。功能缺失研究显示Rab3A参与控制囊泡靶向锚定到活性区域（7）。通过与其直接作用子Rabhillin结合，Rab3A也参与编排突触囊泡的胞吐和内吞之间的偶联（8）。

1. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
2. Geppert, M. et al. (1994) Nature 369, 493-7.
3. Weber, E. et al. (1996) J Biol Chem 271, 6963-71.
4. Yaekura, K. et al. (2003) J Biol Chem 278, 9715-21.
5. Johannes, L. et al. (1994) EMBO J 13, 2029-37.
6. Geppert, M. et al. (1997) Nature 387, 810-4.
7. Leenders, A.G. et al. (2001) Mol Biol Cell 12, 3095-102.
8. Coppola, T. et al. (2001) J Cell Sci 114, 1757-64.

原厂资料：

Specificity / Sensitivity

Rab3A Antibody detects endogenous levels of total Rab3A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu391 of human Rab3A. Antibodies are purified by peptide affinity chromatography.

Background

The Rab family of proteins includes small, monomeric GTPases essential for regulating intracellular vesicle trafficking. Members of the Rab3 subfamily, including Rab3A-3D, are involved in the exocytosis of neurotransmitters and hormones (1). Rab3A is primarily expressed in neurons (2), neuroendocrine cells (such as rat PC-12 cells), and in human pancreatic β cells (3, 4). By acting as a molecular switch between active, GTP-bound Rab3A and the inactive, GDP-bound form, Rab3a inhibits synaptic vesicle and chromaffin granule secretion during late membrane release (5,6). Loss-of-function studies suggest Rab3A is involved in controlling synaptic vesicle targeting and docking at the active zone (7). Through binding to its direct effector Rabphillin, Rab3A also orchestrates the coupling between synaptic vesicle exocytosis and endocytosis (8).

1. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
2. Geppert, M. et al. (1994) Nature 369, 493-7.
3. Weber, E. et al. (1996) J Biol Chem 271, 6963-71.
4. Yaekura, K. et al. (2003) J Biol Chem 278, 9715-21.
5. Johannes, L. et al. (1994) EMBO J 13, 2029-37.
6. Geppert, M. et al. (1997) Nature 387, 810-4.
7. Leenders, A.G. et al. (2001) Mol Biol Cell 12, 3095-102.
8. Coppola, T. et al. (2001) J Cell Sci 114, 1757-64.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.