Phospho-AMPA Receptor 2 (GluA2) (Tyr869/Tyr873/Tyr876) Antibody

• 产品编号：CST-3921S      品牌：CST       原厂货号：3921S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-AMPA Receptor (GluR 2) (Tyr869/Tyr873/Tyr876) Antibody 兔多抗识别内源性的Tyr869、Tyr873或者Tyr876磷酸化的GluR 2蛋白。它也可以识别Tyr880、Tyr884或Tyr887磷酸化的GluR 3蛋白。这些残基与GluR 1或GluR 4中的不保守同源。该多克隆抗体由合成的人类GluR 2蛋白 Tyr869、 Tyr873 和 Tyr876位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。AMPA（α-氨基-3-羟基-5-甲基-4-恶唑酸），kainite和NMDA（N-甲基-D-天冬氨酸）受体是离子型谷氨酸门控离子通道的三个主要的家族。AMPA受体（AMPARs）是由四个亚基（GluR1-4）构成，以同源或异源四聚体的形式介导大多数中枢神经系统的兴奋性传递。AMPARs与突触的形成，稳定和可塑性有关（1）。缺乏GluR2的AMPARs可渗透钙，包含GluR2的AMPARs相反（2）。转录后修饰（剪接，RNA编辑核）和翻译后修饰（糖基化，磷酸化）可以导致结果的多样，可以微调AMPARs的动力学性质。AMPARs酶活性的变化与各种疾病有关，包括阿尔茨海默氏症，肌萎缩性侧索硬化症（ALS），中风，癫痫（1）。Src家族酪氨酸激酶磷酸化AMPA受体GluR 2亚基的Tyr876，从而增加GRIP1 / 2的相互作用，但不影响PICK1。此外，Tyr876对AMPA和NMDA诱导GluR 2内在化是重要的（3）。Tyr869、Tyr873和Tyr876磷酸化位点由CST公司使用PhosphoScan®和CST的磷酸化位点质谱平台发现。GluR2在Tyr869，Tyr873和Tyr876上的磷酸化在缺血大鼠脑组织分离提取物中可见。这些位点从小鼠大脑的酪氨酸磷酸化大规模鉴定中被独立发现（4）。

1. Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77.
2. Cull-Candy, S. et al. (2006) Curr Opin Neurobiol 16, 288-97.
3. Hayashi, T. and Huganir, R.L. (2004) J. Neurosci. 24, 6152-6160.
4. Ballif, B.A. et al. (2008) J. Proteome Res. 7, 311-318.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human, Mouse

Specificity / Sensitivity

Phospho-AMPA Receptor (GluR 2) (Tyr869/Tyr873/Tyr876) Antibody detects endogenous levels of GluR 2 only when phosphorylated at Tyr869, Tyr873 or Tyr876. It may also detect GluR 3 when phosphorylated at the conserved Tyr880, Tyr884 or Tyr887. These residues are not conserved in GluR 1 or GluR 4.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr869, Tyr873 and Tyr876 of human GluR 2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainite- and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). AMPARs that lack GluR 2 are permeable to calcium, in contrast to GluR 2 containing AMPARs (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Activity changes of AMPARs are implicated in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1).Src family tyrosine kinases phosphorylate the GluR 2 subunit of AMPA receptors at Tyr876, which increases the interaction with GRIP1/2 but not PICK1. In addition, Tyr876 is important for AMPA- and NMDA-induced GluR 2 internalization (3).The phosphorylation sites at Tyr869, Tyr873 and Tyr876 were identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of GluR2 at Tyr869, Tyr873 and Tyr876 was observed in extracts isolated from ischemic rat brain. These sites were independently found in a large-scale identification of tyrosine phosphorylation sites from murine brain (4).

1. Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77.
2. Cull-Candy, S. et al. (2006) Curr Opin Neurobiol 16, 288-97.
3. Hayashi, T. and Huganir, R.L. (2004) J. Neurosci. 24, 6152-6160.
4. Ballif, B.A. et al. (2008) J. Proteome Res. 7, 311-318.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.