Bag1 (3.10G3E2) Mouse mAb

• 产品编号：CST-3920S      品牌：CST       原厂货号：3920S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Bag1 (3.10G3E2) Mouse mAb识别内源性的Bag1总蛋白，包括长、中、短亚型。该单克隆抗体用重组全长人Bag1免疫动物而制成。Bag1属于Bcl-2相关athanogene（BAG）家族多功能蛋白质，是从人类分离的六个相关蛋白中的第一个（1，2）。这个广泛表达的蛋白与很多信号分子相互作用（包括Bcl2、HGF受体和Raf1），调节涉及细胞存活、生长和分化的信号通路。Bag1蛋白最常见的功能是抑制促进凋亡的蛋白（2-4）。Bag1也在Raf1信号通路中扮演角色，可以作为转录激活因子结合DNA（4）。Bag1蛋白是其结合物HSP70的了解较多的抑制剂（5）。Bag1羧基末端的BAG结构域与HSP70的ATP酶域相互作用，负向调节热休克蛋白分子伴侣活性（6，7）。Bag1蛋白的多个亚型从一个序列中转录生成，共享一个常见的泛素同源结构域和一个羧基末端Hsp70结合区域，但在长度和细胞定位上有区别。50kDa长的Bag1-L亚型包含一个核定位信号，常在细胞核内发现，激活转录。46kDa中等长度的Bag1-M亚型主要在细胞质中发现，但也可以在与其它蛋白质结合后转位到细胞核。29-33kDa亚型（Bag1-S、Bag-1）主要发现在细胞质中（8）。高表达抗凋亡Bag1蛋白与某些癌症患者增加的存活率相关，导致研究者研究Bag1蛋白潜在的抗肿瘤作用（9）。

1. Kudoh, M. et al. (2002) Cancer Res 62, 1904-9.
2. Takayama, S. et al. (1995) Cell 80, 279-84.
3. Bardelli, A. et al. (1996) EMBO J 15, 6205-12.
4. Wang, H.G. et al. (1996) Proc Natl Acad Sci USA 93, 7063-8.
5. Takayama, S. et al. (1997) EMBO J 16, 4887-96.
6. Bimston, D. et al. (1998) EMBO J 17, 6871-8.
7. Briknarová, K. et al. (2001) Nat Struct Biol 8, 349-52.
8. Knee, D.A. et al. (2001) J Biol Chem 276, 12718-24.
9. Millar, E.K. et al. (2009) Br J Cancer 100, 123-33.

原厂资料：

Specificity / Sensitivity

Bag1 (3.10G3E2) Mouse mAb detects endogenous levels of total Bag1 protein, including long, medium and short isoforms.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant full length human Bag1.

Background

Bag1 belongs to the Bcl-2 associated athanogene (BAG) family of multifunctional proteins and was the first of six related proteins isolated from humans (1,2). This widely expressed protein interacts with a number of signaling molecules (including Bcl2, HGF receptor and Raf1) as it regulates signaling molecules in pathways involving cell survival, growth and differentiation. The most common role played by Bag1 protein is as an inhibitor of proteins favoring apoptosis (2-4). Bag1 also plays a role in Raf1 signaling and binds DNA as a transcription activator (4). Bag1 protein is a well-characterized inhibitor of its binding partner HSP70 (5). A conserved carboxy-terminal BAG domain within Bag1 interacts with the ATPase domain of HSP70 to negatively regulate heat shock protein chaperone activity (6,7). The multiple isoforms of Bag1 protein generated from a single transcript share a common ubiquitin homology domain and a carboxy-terminal Hsp70 binding region but differ in length and cellular localization. The 50 kDa long (Bag1-L) isoform also contains a nuclear localization signal and is often found in the nucleus where it activates transcription. The 46 kDa intermediate (Bag1-M) isoform is found mainly in the cytoplasm but can also translocate to the nucleus when associated with other proteins. The shorter 29-33 kDa isoforms (Bag1-S, Bag-1) are found primarily in the cytoplasm (8). High expression of the anti-apoptotic Bag1 protein correlates with increased survival in patients with particular forms of cancer, leading researchers to study possible therapeutic roles for Bag1 protein (9).

1. Kudoh, M. et al. (2002) Cancer Res 62, 1904-9.
2. Takayama, S. et al. (1995) Cell 80, 279-84.
3. Bardelli, A. et al. (1996) EMBO J 15, 6205-12.
4. Wang, H.G. et al. (1996) Proc Natl Acad Sci USA 93, 7063-8.
5. Takayama, S. et al. (1997) EMBO J 16, 4887-96.
6. Bimston, D. et al. (1998) EMBO J 17, 6871-8.
7. Briknarová, K. et al. (2001) Nat Struct Biol 8, 349-52.
8. Knee, D.A. et al. (2001) J Biol Chem 276, 12718-24.
9. Millar, E.K. et al. (2009) Br J Cancer 100, 123-33.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.