Phospho-p56Dok-2 (Tyr351) Antibody

• 产品编号：CST-3911S      品牌：CST       原厂货号：3911S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-p56Dok-2 (Tyr351)兔多抗能检测酪氨酸（351位点）磷酸化的转染p56Dok-2蛋白水平。该抗体不与其他酪氨酸磷酸化的p62Dok蛋白家族成员发生交叉反应。该多克隆抗体是通过用与人源p56Dok-2蛋白酪氨酸（351位点）周围残基相一致的磷酸化的合成肽段免疫动物后获得的。该抗体经蛋白A和肽亲和层析纯化。停泊蛋白是酪氨酸激酶的底物，酪氨酸激酶能聚集并装配特异性信号转导分子。p62dok蛋白家族一共有五个成员，p62Dok (Dok-1), p56Dok-2 (Dok-2, or DoK-R), Dok-3, Dok-4 and Dok-5 (1-3)，主要特点是胺端PH结构域、中心PTB结构域和许多酪氨酸磷酸化的潜在位点。p56Dok-2蛋白的酪氨酸磷酸化发生在依赖于多种刺激因子刺激细胞，或细胞内致癌酪氨酸激酶如v-Src、Bcr-Abl改变其蛋白结构(3-5)。由于存在一些信号传递结构域(PH, PTB 结构域, 酪氨酸残基和脯氨酸富集区域)，人们推测p62dok蛋白家族充当停泊蛋白，将RTKs和信号转导途径连接起来。据推测p56Dok-2是T细胞中细胞因子诱导增值的负调节因子(5)。酪氨酸（351位点）磷酸化的p56Dok-2蛋白能调节与Nck蛋白SH2结构域的关联(4)。

1. Master, Z. et al. (2001) EMBO J. 20, 5919-5928.
2. Grimm, J. et al. (2001) J. Cell. Biol. 154, 345-354.
3. Cristofano, A. D. et al. (1998) J. Biol. Chem. 273, 4827-4830.
4. Jones, N. and Dumont, D.J. (1999) Curr. Biol. 9, 1057-1060.
5. Nemorin, J.G. and Duplay, P. (2000) J. Biol. Chem. 275, 14590-14597.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse

Specificity / Sensitivity

Phospho-p56Dok-2 (Tyr351) Antibody detects transfected levels of p56Dok-2 only when phosphorylated at tyrosine 351. The antibody does not cross-react with other tyrosine phosphorylated p62Dok family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr351 of mouse p56Dok-2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Docking proteins are substrates of tyrosine kinases that function in the recruitment and assembly of specific signal transduction molecules. There are five members in the p62dok family, p62Dok (Dok-1), p56Dok-2 (Dok-2, or DoK-R), Dok-3, Dok-4 and Dok-5 (1-3), characterized by the presence of an amino-terminal PH domain, a central PTB domain and numerous potential sites of tyrosine phosphorylation. Tyrosine phosphorylation of p56Dok-2 occurs upon stimulation of cells with a variety of stimuli, or in cells transformed by oncogenic tyrosine kinases such as v-Src and Bcr-Abl (3-5). Based on the presence of several signaling domains (PH, PTB domain, tyrosine residue and proline-rich regions), it has been proposed that the p62dok family act as docking proteins that link RTKs to signal transduction pathways. p56Dok-2 has been proposed to be a negative regulator of cytokine-induced proliferation in T cells (5). Phosphorylated Tyr351 of p56Dok-2 mediates an association with the SH2 domain of Nck (4).

1. Master, Z. et al. (2001) EMBO J. 20, 5919-5928.
2. Grimm, J. et al. (2001) J. Cell. Biol. 154, 345-354.
3. Cristofano, A. D. et al. (1998) J. Biol. Chem. 273, 4827-4830.
4. Jones, N. and Dumont, D.J. (1999) Curr. Biol. 9, 1057-1060.
5. Nemorin, J.G. and Duplay, P. (2000) J. Biol. Chem. 275, 14590-14597.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.