GAPDH (14C10) Rabbit mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-3906S      品牌：CST       原厂货号：3906S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

GAPDH (14C10) Rabbit mAb 兔单抗(Alexa Fluor® 488 Conjugate)用于检测内源性GAPDH总蛋白水平。该单抗是通过合成对应于人GAPDH蛋白羧基端序列的肽段免疫动物获得。本抗体是在最适条件下以2-6的F/P比例与Alexa Fluor® 488染料相连形成的。本抗体与Alexa Fluor® 488荧光染料结合，并且经过了人源细胞的荧光免疫实验和流式实验验证。本抗体的交互反应应与非交联的GAPDH (14C10) Rabbit mAb兔单抗 #2118相同。甘油醛－3－磷酸脱氢酶（GAPDH）在糖酵解过程中将3-磷酸甘油醛进一步磷酸化。虽然在不同组织中的表达水平有差异，但GAPDH普遍被认为是一个组成型表达的持家蛋白。正是由于这个原因，GAPDH的mRNA和蛋白通常在对其它有变化的靶蛋白测定实验时作为对照。最近的研究阐明了GAPDH在细胞凋亡（2），基因表达（3）和核转运（4）过程中的作用。GAPDH还可能对神经退行性疾病如亨廷顿和阿尔茨海默病的发生起着作用（4,5）。

1. Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
2. Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
3. Zheng, L. et al. (2003) Cell 114, 255-66.
4. Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
5. Wang, Q. et al. (2005) FASEB J. 19, 869-71.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Pig

Specificity / Sensitivity

GAPDH (14C10) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total GAPDH protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH. This antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated GAPDH (14C10) Rabbit mAb #2118.

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3) and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).

1. Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
2. Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
3. Zheng, L. et al. (2003) Cell 114, 255-66.
4. Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
5. Wang, Q. et al. (2005) FASEB J. 19, 869-71.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide, 2 mg/ml BSA. Store at 4°C. Protect from light.
Do not freeze.