Gα(z) Antibody

• 产品编号：CST-3904S      品牌：CST       原厂货号：3904S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Gα(z)抗体识别内源性的全部Gα(z)蛋白。该多克隆抗体用与人类Gα(z)蛋白氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和肽亲和层析纯化而得。异三聚鸟嘌呤核苷酸结合蛋白（G蛋白）由α、β和γ亚基构成，介导激素、神经递质、趋化因子和感觉刺激的作用。到今天为止，根据结构和功能的相似性，超过20个亚基分为四个家族，分别是Gα(s), Gα(i/o), Gα(q)和Gα(12)（1,2）。Gα(q)/Gα(11)的Tyr356磷酸化对G蛋白激活是至关重要的，因为苯丙氨酸替代Tyr356改变了Gα与受体的相互作用，并消除了配体诱导的IP3的形成（3）。Gα(z)与其它G蛋白不同，它缺乏百日咳毒素的ADP-ribosylation共有位点，因此可能在对抗毒素的信号转导通路中发挥一定所用，类似磷脂酶C（4）。Gα(z)被蛋白磷酸酶C（PKC）在Ser27处磷酸化激活。

1. Offermanns, S. (2001) Oncogene 20, 1635-42.
2. Pierce, K.L. et al. (2002) Nat Rev Mol Cell Biol 3, 639-50.
3. Umemori, H. et al. (1997) Science 276, 1878-81.
4. Fong, H.K. et al. (1988) Proc Natl Acad Sci USA 85, 3066-70.
5. Fields, T.A. and Casey, P.J. (1995) J Biol Chem 270, 23119-25.
6. Ho, M.K. et al. Biol Signals Recept 9, 21-8.

原厂资料：

Specificity / Sensitivity

Gα(z) Antibody detects endogenous levels of total Gα(z) protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human Gα(z). Antibodies are purified using protein A and peptide affinity chromatography.

Background

Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3).Gα(z) stands out from other G proteins because it lacks an ADP-ribosylation consensus site for pertussis toxin, giving it a possible role in signal transduction pathways resistant to the toxin, such as phospholipase C (4). Gα(z) is phosphorylated and activated by protein kinase C (PKC) at Ser27 (5,6).

1. Offermanns, S. (2001) Oncogene 20, 1635-42.
2. Pierce, K.L. et al. (2002) Nat Rev Mol Cell Biol 3, 639-50.
3. Umemori, H. et al. (1997) Science 276, 1878-81.
4. Fong, H.K. et al. (1988) Proc Natl Acad Sci USA 85, 3066-70.
5. Fields, T.A. and Casey, P.J. (1995) J Biol Chem 270, 23119-25.
6. Ho, M.K. et al. Biol Signals Recept 9, 21-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.