AFP (3H8) Mouse mAb

• 产品编号：CST-3903S      品牌：CST       原厂货号：3903S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

AFP (3H8)小鼠单抗检测内源性的总AFP 蛋白水平.AFP单克隆抗体由人源AFP重组蛋白片段免疫动物产生.Cell Signaling Technology Alpha-fetoprotein (AFP)是一个在哺乳动物胎儿肝脏，卵黄囊,和胃肠道中发现的65kDa的糖蛋白。AFP在成体细胞中表达较低，而在成体肝癌细胞中异常表达（1,2）。抑癌基因p53和β-catenin都涉及到对AFP的表达调控。在正常成体细胞中，p53和AFP基因的抑制区结合，因此阻断其转录。P53和β-catenin的突变都与AFP的异常表达相关。研究显示AFP在血清中表达升高预示着肝细胞癌（3）。

1. Meier, V. et al. (2006) Comp Hepatol 5, 2.
2. Warnes, T.W. and Smith, A. (1987) Baillieres Clin Gastroenterol 1, 63-89.
3. Peng, S.Y. et al. (2004) Int J Cancer 112, 44-50.

原厂资料：

Specificity / Sensitivity

AFP (3H8) Mouse mAb detects endogenous levels of total AFP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human

Background

Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma (3).

1. Meier, V. et al. (2006) Comp Hepatol 5, 2.
2. Warnes, T.W. and Smith, A. (1987) Baillieres Clin Gastroenterol 1, 63-89.
3. Peng, S.Y. et al. (2004) Int J Cancer 112, 44-50.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.