PARN (P620) Antibody

• 产品编号：CST-3899S      品牌：CST       原厂货号：3899S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PARN (P620) Antibody检测内源性PARN总蛋白水平。通过人工合成人源PARN蛋白Pro620位点附近序列相应多肽片段来免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。细胞内mRNAs的水平由mRNA稳定性、合成率和降解率共同控制。poly(A)尾的存在与否和长度与mRNA稳定性有关(1)。由外切核酸导致的poly(A)尾缩短是许多真核中引起mRNAs降解的过程(2)。Poly(A)-specific ribonuclease (PARN)是负责去腺苷化的起始和由核酸外切所致的mRNA转录本缩短的酶。通过一个进化保守机制，在胚胎发育早期PARN也翻译性的沉默选择性mRNAs(3)。在许多哺乳动物组织中PARN蛋白持续性表达，并且在基因表达的转录后调控里起着重要作用(4)。

1. Morales, J. et al. (1997) J Biol Chem 272, 6607-13.
2. Wilson, T. and Treisman, R. (1988) Nature 336, 396-9.
3. Sachs, A.B. et al. (1997) Cell 89, 831-8.
4. Fritz, D.T. et al. (2004) Cell Biochem Biophys 41, 265-78.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog

Specificity / Sensitivity

PARN (P620) Antibody detects endogenous levels of total PARN protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequences surrounding Pro620 of human PARN. Antibodies are purified by Protein A and peptide affinity chromatography.

Background

Cellular levels of mRNAs are controlled by mRNA stability, the rate of synthesis and the rate of degradation. The presence and length of the poly(A) tail has been associated with mRNA stability (1). Exonucleolytic shortening of the poly(A) tail is the process that initiates the decay of many eukaryotic mRNAs (2). Poly(A)-specific ribonuclease (PARN) is the enzyme responsible for initiation of deadenylation and exonucleolytic shortening of mRNA transcripts. Through an evolutionarily conserved mechanism, PARN also translationally silences selective mRNAs during early embryonic development (3). PARN is constitutively expressed in most mammalian tissues and plays a critical role in the post-transcriptional control of gene expression (4).

1. Morales, J. et al. (1997) J Biol Chem 272, 6607-13.
2. Wilson, T. and Treisman, R. (1988) Nature 336, 396-9.
3. Sachs, A.B. et al. (1997) Cell 89, 831-8.
4. Fritz, D.T. et al. (2004) Cell Biochem Biophys 41, 265-78.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody