Glycogen Synthase Antibody

• 产品编号：CST-3893S      品牌：CST       原厂货号：3893S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Glycogen Synthase Antibody用于检测内源性肌肉糖元合酶总蛋白水平多克隆抗体通过用对应于人源糖元合酶蛋白序列的合成肽免疫动物制备而得，抗体经蛋白A和肽段亲和层析纯化制得。糖元合成酶（GS）是糖元合成调节中的关键酶。哺乳动物有两种GS同工酶：肌肉GS和肝脏GS（1－2）。肌肉GS在多个组织中都有表达，而肝脏GS似乎是组织特异性的（3）。GS受到多个位点磷酸化的调控，同时也受变构配体结合的调控。磷酸化会抑制GS活性，但活性可被变异配体6-磷酸葡萄糖结合后恢复，6-磷酸葡萄糖可以诱导GS的去磷酸化从而激活。

1. Browner, M.F. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 1443-1447.
2. Bai, G. et al. (1990) J. Biol. Chem. 265, 7843-7848.
3. Roach, P.J. et al. (1998) J. Basic Clin. Physiol. Pharmacol. 9, 139-151.
4. Villar-Palasi, C. and Guinovart, J.J. (1997) FASEB J. 11, 544-558.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Horse

Specificity / Sensitivity

Glycogen Synthase Antibody detects endogenous levels of total muscle glycogen synthase protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human glycogen synthase. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Glycogen synthase (GS) is a key enzyme in the regulation of glycogen synthesis. There are two main isoforms of mammalian GS: the muscle and the liver isoform (1-2). Muscle GS is expressed in several tissues, and liver GS appears to be tissue specific (3). GS is regulated by multi-site phosphorylation and by the binding of allosteric ligands. Phosphorylation leads to inactivation of GS, but activity is restored by the binding of the allosteric activator glucose-6-phosphate, which induces its dephosphorylation and activation (4).

1. Browner, M.F. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 1443-1447.
2. Bai, G. et al. (1990) J. Biol. Chem. 265, 7843-7848.
3. Roach, P.J. et al. (1998) J. Basic Clin. Physiol. Pharmacol. 9, 139-151.
4. Villar-Palasi, C. and Guinovart, J.J. (1997) FASEB J. 11, 544-558.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.