HS1 (D83A8) XP? Rabbit mAb (Human Specific)

• 产品编号：CST-3890P      品牌：Cell Signaling Technology       原厂货号：3890P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HS1 (D83A8) XP® Rabbit mAb 兔单抗(特异性适用于人)能够检测内源HS1蛋白总体水平。据推算HS1的分子量是54 kDa，但是在SDS-PAGE胶上显示出80 kDa大小。此单克隆抗体是通过合成人源对应的HS1 Pro310位点周围的肽段来免疫动物而获得。HS1 (HCLS1, LckBP1, p75) 是蛋白激酶底物，只在造血组织和细胞中表达(1,2)。HS1包含四个皮肌动蛋白重复和单个SH3域(2)。此胞内蛋白在免疫受体的激活下磷酸化, 促进HS1被招募到免疫突触(3-5)。磷酸化的HS1被用于调节肌动蛋白的动态并为其它信号分子提供对接位点如Vav1 和 PLCγ1 (6)。HS1在血小板活化过程中也发挥了重要的作用(7)。

1. Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
2. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
3. Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
4. Hata, D. et al. (1994) Immunol Lett 40, 65-71.
5. Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
6. Gomez, T.S. et al. (2006) Immunity 24, 741-52.
7. Kahner, B.N. et al. (2007) Blood 110, 2449-56.

原厂资料：

Specificity / Sensitivity

HS1 (D83A8) XP® Rabbit mAb (Human Specific) detects endogenous levels of total HS1 protein. HS1 has a calculated size of 54 kDa, but has an apparent molecular weight of 80 kDa on SDS-PAGE gels.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro310 of human HS1.

Background

HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).

1. Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
2. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
3. Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
4. Hata, D. et al. (1994) Immunol Lett 40, 65-71.
5. Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
6. Gomez, T.S. et al. (2006) Immunity 24, 741-52.
7. Kahner, B.N. et al. (2007) Blood 110, 2449-56.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.