Caspase-1 (D7F10) Rabbit mAb

• 产品编号：CST-3866S      品牌：CST       原厂货号：3866S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Caspase-1 (D7F10) Rabbit mAb 兔单抗能够检测内源性水平全长人Caspase-1蛋白。通过过表达，有活性的p20亚基可被检测到。该单克隆抗体是采用合成的人源caspase-1蛋白p20亚基相对应的肽段免疫动物而制备的。Caspase-1，又称白介素-1ß转化酶(ICE/ICEα)，是一种 I 类半胱氨酸蛋白酶，I类半胱氨酸蛋白酶还包括caspases -4、-5、-11 和-12。Caspase-1 能够将炎症细胞因子如pro-IL-1ß 和干扰素-γ诱导因子 (IL-18) 剪切成成熟蛋白 (1,2)。与其它的 caspases 一样， caspase-1通过蛋白水解被激活，酶原产生一个由两个活性亚单元p20和 p10组成的四聚体。Caspase-1具有巨大的N-末端前体域，此区域包含一个caspase 招募区域(CARD)。过表达caspase-1 能诱导凋亡(3)。Caspase-1 缺失的小鼠，在凋亡方面没有严重缺陷，但是却在pro-IL-1β 蛋白的成熟过程中有严重缺陷，而且这个小鼠对内毒素有抗性(4,5)。研究已经证实 Caspase-1 至少有六种形式，包括caspase-1 α、β、γ、δ、ε和ζ (6)。大多数caspase-1 (α，β，γ和 δ) 产生的产物在 30-48 kDa之间，并通过过表达诱导凋亡。Caspase-1ε 通常只包含p10亚单元，是没有诱导凋亡活性的显性失活蛋白。caspase-1中广泛表达的ζ形式诱导凋亡，并且比较α形式，其在N-末端缺少39个氨基酸残基(6)。Caspase-1激活的发生是通过一个特殊的“炎症小体”的多聚分子平台，此中包含 caspase-5、Pycard/Asc和 NALP1 (7)。

1. Thornberry, N.A. et al. (1992) Nature 356, 768-74.
2. Martinon, F. and Tschopp, J. (2004) Cell 117, 561-74.
3. Miura, M. et al. (1993) Cell 75, 653-60.
4. Kuida, K. et al. (1995) Science 267, 2000-3.
5. Li, P. et al. (1995) Cell 80, 401-11.
6. Feng, Q. et al. (2004) Genomics 84, 587-91.
7. Martinon, F. et al. (2002) Mol Cell 10, 417-26.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Caspase-1 (D7F10) Rabbit mAb detects endogenous levels of full length human Caspase-1. The activated p20 subunit was detected by over-expression.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues within the p20 subunit of human caspase-1.

Background

Caspase-1, or interleukin-1ß converting enzyme (ICE/ICEα), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1ß and interferon-γ inducing factor (IL-18) into their mature forms (1,2). Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis (3). Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1β and are resistant to endotoxic shock (4,5). At least six caspase-1 isoforms have been identified, including caspase-1 α, β, γ, δ, ε and ζ (6). Most caspase-1 isoforms (α, β, γ and δ) produce products between 30-48 kDa and induce apoptosis upon over-expression. Caspase-1 ε typically contains only the p10 subunit, does not induce apoptosis and may act as a dominant negative. The widely expressed ζ isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the α isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform designated the "inflammasome" that includes caspase-5, Pycard/Asc, and NALP1 (7).

1. Thornberry, N.A. et al. (1992) Nature 356, 768-74.
2. Martinon, F. and Tschopp, J. (2004) Cell 117, 561-74.
3. Miura, M. et al. (1993) Cell 75, 653-60.
4. Kuida, K. et al. (1995) Science 267, 2000-3.
5. Li, P. et al. (1995) Cell 80, 401-11.
6. Feng, Q. et al. (2004) Genomics 84, 587-91.
7. Martinon, F. et al. (2002) Mol Cell 10, 417-26.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.