IL-1RA (20D8) Mouse mAb

• 产品编号：CST-3865S      品牌：CST       原厂货号：3865S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IL-1RA (20D8) Mouse mAb能够检测内源IL-1RA蛋白。此抗体不与其它相关的蛋白交叉反应。此单克隆抗体是通过融合的人IL-1RA蛋白来免疫动物而获得。白介素1(IL-1)超家族中的最基本的成员包括促炎症因子IL-1α和IL-1β, 另外的第三个蛋白是作为IL-1受体拮抗剂(IL-1RA)。最近，至少有六个相似的蛋白得到了鉴定, 包括与IL-1RA (IL1F5)同源的蛋白。有三个得到很好的鉴定的蛋白(IL-1a, IL-1b and IL-1RA)主要是在巨噬细胞、单核细胞核树突状细胞中表达。IL-1a 和 IL-1b 作为潜在的炎症因子并帮助调节寄主抵抗和免疫应答(1)。结合这些促炎症因子到 IL-1受体招募转换器蛋白如IRAK到受体上。磷酸化这些接头蛋白促使下游与免疫应答相关的信号通路(2)。IL-1a和IL-1b表达的改变与很多人类的疾病相关，包括阿尔茨海默病、类风湿关节炎、牛皮癣和各种癌症(3,4)。 IL-1RA作为抗炎症因子结合到IL-1受体上从而限制炎症反应(5)。因为它在调节炎症反应中发挥了重要的调节作用,重组的IL-1RA是一些疾病中的治疗靶标，如类风湿性关节炎。 相反，突变相应的IL-1RA基因可能是与某些癌症更容易发展有关系(6)。

1. Pelegrin, P. (2008) Drug News Perspect 21, 424-33.
2. Ringwood, L. and Li, L. (2008) Cytokine 42, 1-7.
3. Griffin, W.S. and Mrak, R.E. (2002) J Leukoc Biol 72, 233-8.
4. Kamangar, F. et al. (2006) Cancer Epidemiol Biomarkers Prev 15, 1920-8.
5. Arend, W.P. (2002) Cytokine Growth Factor Rev 13, 323-40.
6. Sehouli, J. et al. (2002) Anticancer Res 22, 3421-4.

原厂资料：

Specificity / Sensitivity

IL-1RA (20D8) Mouse mAb detects endogenous IL-1RA protein. It does not cross-react with other related proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IL-1RA protein.

Background

The founding members of the interleukin-1 (IL-1) superfamily include pro-inflammatory cytokines IL-1α and IL-1β, and a third protein that acts as an IL-1 receptor antagonist (IL-1RA). At least six similar proteins have been recently identified, including a homolog of IL-1RA (IL1F5). The three better-characterized proteins (IL-1a, IL-1b and IL-1RA) are mainly expressed in macrophages, monocytes, and dendritic cells. IL-1a and IL-1b act as potent inflammatory cytokines that help regulate host defense and immune responses (1). Binding of these pro-inflammatory cytokines to an IL-1 receptor recruits adapter proteins (such as IRAK) to the receptor. Phosphorylation of these adaptor proteins promotes downstream signaling cascades associated with the immune response (2). Altered expression of both IL-1a and IL-1b is associated with an extensive list of human disorders, including Alzheimer's disease, rheumatoid arthritis, psoriasis and various forms of cancer (3,4). IL-1RA acts as an anti-inflammatory cytokine, binding the IL-1 receptor to limit the response to inflammation (5). Because it plays a key role in regulating the inflammatory response, recombinant IL-1RA is a therapeutic agent used in the treatment of diseases such as rheumatoid arthritis. Alternatively, mutation of the corresponding IL-1RA gene may be associated with susceptibility to the development of specific cancers (6).

1. Pelegrin, P. (2008) Drug News Perspect 21, 424-33.
2. Ringwood, L. and Li, L. (2008) Cytokine 42, 1-7.
3. Griffin, W.S. and Mrak, R.E. (2002) J Leukoc Biol 72, 233-8.
4. Kamangar, F. et al. (2006) Cancer Epidemiol Biomarkers Prev 15, 1920-8.
5. Arend, W.P. (2002) Cytokine Growth Factor Rev 13, 323-40.
6. Sehouli, J. et al. (2002) Anticancer Res 22, 3421-4.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody