Phospho-FRS2-α (Tyr436) Antibody

• 产品编号：CST-3861S      品牌：CST       原厂货号：3861S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-FRS2-alpha (Tyr436) Antibody兔多抗能检测内源性酪氨酸（436位点）磷酸化的FRS2-α蛋白水平。该抗体不与无关的酪氨酸磷酸化蛋白发生交叉反应。该多克隆抗体通过使用与人源FRS2-α蛋白酪氨酸（436位点）周围残基相一致的磷酸化的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。成纤维细胞生长因子受体底物 (FRS2,也被称为Suc-相关的神经营养因子诱导的酪氨酸磷酸化靶点或SNT）参与将信号从成纤维细胞生长因子受体 (FGFR)向细胞外传送。FGFR的活化导致FRS2的酪氨酸磷酸化 (1)。目前已鉴定出两个FRS2家族成员，FRS2-alpha (SNT1) 和 FRS2-beta (SNT2) (2)，这两种蛋白都被这些RTKs磷酸化。一旦这些蛋白被磷酸化，它们就会聚集含有SH2结构域的蛋白，包括Grb2 and SHP-2 (3,4),以此调节下游信号传递。酪氨酸（436位点）是SHP-2能有效聚集所必需的(5),然而酪氨酸（196位点）的作用是抑制Grb2-Sos复合物(6)。

1. Kouhara, H. et al. (1997) Cell 89, 693-702.
2. Ong, S. H. et al. (2000) Mol. Cell. Biol. 20, 979-989.
3. Kontaridis, M. I. et al. (2002) Mol. Cell. Biol. 22, 3875-3891.
4. Xu, H. and Goldfarb, M. (2001) J. Biol. Chem. 276, 13049-13056.
5. Hadari, Y. R. et al. (1998) Mol. Cell. Biol. 18, 3966-3973.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

Phospho-FRS2-alpha (Tyr436) Antibody detects endogenous levels of FRS2-alpha only when phosphorylated at tyrosine 436. The antibody does not cross-react with unrelated tyrosine-phosphorylated proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr436 of human FRS2-alpha. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Fibroblast growth factor receptor substrate 2 (FRS2, also called Suc-associated neurotrophic factor-induced tyrosine-phosphorylated target or SNT) participates in the transmission of extracellular signals from the fibroblast growth factor receptor (FGFR). Activation of the FGFR leads to tyrosine phosphorylation of FRS2 (1). Two FRS2 family members have been identified, FRS2-alpha (SNT1) and FRS2-beta (SNT2) (2), which are phosphorylated by these RTKs. Once they are phosphorylated, they recruit SH2 domain-containing proteins including Grb2 and SHP-2 (3,4), mediating downstream signaling. Tyr436 is required for efficient SHP-2 recruitment (5), whereas Tyr196 functions as a docking site for Grb2-Sos complexes (6).

1. Kouhara, H. et al. (1997) Cell 89, 693-702.
2. Ong, S. H. et al. (2000) Mol. Cell. Biol. 20, 979-989.
3. Kontaridis, M. I. et al. (2002) Mol. Cell. Biol. 22, 3875-3891.
4. Xu, H. and Goldfarb, M. (2001) J. Biol. Chem. 276, 13049-13056.
5. Hadari, Y. R. et al. (1998) Mol. Cell. Biol. 18, 3966-3973.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.