PLCγ Antibody Sampler Kit

• 产品编号：CST-3860S      品牌：Cell Signaling Technology       原厂货号：3860S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

每个PLCγ Antibody Sampler Kit试剂盒中的抗体均识别其对应的蛋白质。各个抗体均不与其它PLC发生交叉反应。PLCγ1单克隆抗体用与人类PLCγ1中Leu1220位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成，多克隆抗体用与人类PLCγ1和PLCγ2对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。PLCγ Antibody Sampler Kit试剂盒提供了一个经济的手段来研究磷酸化的或全部的PLCγ水平。该试剂盒包含足够每个抗体完成四次Western blots的一抗和二抗。磷酸肌醇特异性磷脂酶C（PLC）在跨膜信号转导中扮演明显的作用。在对激素、生长因子和神经递质反应的过程中，PLC水解4,5-二磷酸磷酸酰肌醇，产生两个第二信使：1,4,5-三磷酸肌醇（IP3）和甘油二酯（DAG）（1）。至少四个PLC家族已被鉴定，分别为PLC-α、PLC-β、PLC-γ和PLC-δ。磷酸化是调节PLC活性的重要机制之一。PLCγ被受体和非受体酪氨酸激酶激活（2）。PLCγ与EGF和PDGF受体形成复合物，导致PLCγ的Tyr771、783和1245位点磷酸化（3）。Syk对Tyr783的磷酸化激活PLCγ1的酶活性（4）。PLCγ2参与B细胞抗原依赖的信号转导和血小板胶原依赖的信号转导。Btk或Lck对Tyr753、759、1197和1217的磷酸化与PLCγ2的活性相关（5,6）

1. Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509.
2. Margolis, B. et al. (1989) Cell 57, 1101-7.
3. Kim, H.K. et al. (1991) Cell 65, 435-41.
4. Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7.
5. Watanabe, D. et al. (2001) J Biol Chem 276, 38595-601.
6. Ozdener, F. et al. (2002) Mol Pharmacol 62, 672-9.

原厂资料：

Specificity / Sensitivity

Each antibody in the PLCγ Antibody Sampler Kit detects endogenous levels of its target protein. The antibodies do not cross react with other PLCs.

Source / Purification

PLCγ1 monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1220 of human PLCγ1 protein.Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding human PLCγ1 and PLCγ2. Antibodies are purified by protein A and peptide affinity chromatography.

Description

PLCγ Antibody Sampler Kit provides an economical means of analyzing phospho and total PLCγ levels. PLCγ Antibody Sampler Kit contains enough primary and secondary antibodies to perform four western blot experiments with each antibody.

Background

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1245 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6).

1. Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509.
2. Margolis, B. et al. (1989) Cell 57, 1101-7.
3. Kim, H.K. et al. (1991) Cell 65, 435-41.
4. Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7.
5. Watanabe, D. et al. (2001) J Biol Chem 276, 38595-601.
6. Ozdener, F. et al. (2002) Mol Pharmacol 62, 672-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibodies.