ILK1 (4G9) Rabbit mAb

• 产品编号：CST-3856S      品牌：CST       原厂货号：3856S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ILK1 (4G9)兔源单抗检测内源性总的ILK1 蛋白，该抗体可能与ILK2交叉反应。单克隆抗体由合成肽段免疫动物产生，该肽段对应人ILK1的C末端.整合素连锁的激酶（ILKs）偶联整合素和生长因子到下游通路涉及细胞存活，细胞周期调控，细胞与细胞粘附和细胞运动（1）。ILK起着支架作用，通过与整合素, PINCH (通过 Nck2连接 ILK到 RTKs), CH-ILKBP 和 affixin相互作用，桥连胞外基质（ECM）和生长因子受体到肌动蛋白细胞骨架(1)。ILK磷酸化AKT丝氨酸473，GSK-3的丝氨酸9，还有肌球蛋白轻链2（MLC2）的丝氨酸18/苏氨酸19，以及affixin（2-5）。这些磷酸化是调控靶标活性的关键步骤。ILK活性被PI3激酶刺激，并被肿瘤抑制因子PTEN和PP2C蛋白磷酸酶，ILKAP负向调控(1,3,6)。已经有报道在激活环总的保守的丝氨酸343残基在ILK1的激活中起关键作用（2）。

1. Wu, C. and Dedhar, S. (2000) J. Biol. Chem. 155, 505-510.
2. Persad, S. et al. (2001) J. Biol. Chem. 276, 27462-27469.
3. Persad, S. et al. (2000) J. Cell Biol. 153, 1161-1173.
4. Deng, J.T. et al. (2001) J. Biol. Chem. 276, 16365-16373.
5. Yamaji, S. et al. (2001) J. Cell Biol. 153, 1251-1264.
6. Morimoto, A.M. et al. (2000) Oncogene 19, 200-209.

原厂资料：

Specificity / Sensitivity

ILK1 (4G9) Rabbit mAb detects endogenous levels of total ILK1 protein. The antibody may cross-react with ILK2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human ILK1.

Background

Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1 (2).

1. Wu, C. and Dedhar, S. (2000) J. Biol. Chem. 155, 505-510.
2. Persad, S. et al. (2001) J. Biol. Chem. 276, 27462-27469.
3. Persad, S. et al. (2000) J. Cell Biol. 153, 1161-1173.
4. Deng, J.T. et al. (2001) J. Biol. Chem. 276, 16365-16373.
5. Yamaji, S. et al. (2001) J. Cell Biol. 153, 1251-1264.
6. Morimoto, A.M. et al. (2000) Oncogene 19, 200-209.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.