MMP-9 Antibody

• 产品编号：CST-3852S      品牌：CST       原厂货号：3852S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MMP-9抗体检测全长（酶原，92kDa）和剪切的（活性酶，84kDa）MMP-9.多克隆抗体由合成肽段免疫动物产生，该肽段对应人mmp-9的107-117位氨基酸残基，抗体由蛋白A和肽段亲和层析纯化.基质金属蛋白酶（MMP）是一个家族的蛋白酶，靶标多种胞外蛋白，包括其他蛋白酶，生长因子，细胞表面受体和粘附分子（1），在这些家族成员中，MMP-2, MMP-3, MMP-7和 MMP-9在胚胎发育、伤口恢复、肿瘤侵袭、血管生成、致癌和凋亡中已经被认为作为重要因子带有对正常组织重塑（2-4）。MMP活性与癌症发展成正相关（2）。MMP调控的一种机制是转录调控（5）。一旦合成，MMP以潜在的酶原形式存在，MMP活性最大化需要溶蛋白性裂解，通过从全长蛋白释放抑制性前肽结构域而产生活性MMPs（5）.

1. McCawley, L.J. and Matrisian, L.M. (2001) Curr Opin Cell Biol 13, 534-40.
2. Coussens, L.M. et al. (2002) Science 295, 2387-92.
3. Sternlicht, M.D. et al. (1999) Cell 98, 137-46.
4. Vu, T.H. et al. (1998) Cell 93, 411-22.
5. Nagase, H. et al. (1990) Biochemistry 29, 5783-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

MMP-9 Antibody detects full length (proenzyme, 92 kDa) and cleaved (active enzyme, 84 kDa) MMP-9.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues between 107-117 of human MMP-9. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).

1. McCawley, L.J. and Matrisian, L.M. (2001) Curr Opin Cell Biol 13, 534-40.
2. Coussens, L.M. et al. (2002) Science 295, 2387-92.
3. Sternlicht, M.D. et al. (1999) Cell 98, 137-46.
4. Vu, T.H. et al. (1998) Cell 93, 411-22.
5. Nagase, H. et al. (1990) Biochemistry 29, 5783-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.