GABA(B)R2 Antibody

• 产品编号：CST-3839S      品牌：CST       原厂货号：3839S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GABA(B)R2 Antibody 兔多抗识别内源性的总GABA(B)R2蛋白。该多克隆抗体由合成的人类GABA(B)R2蛋白氨基酸序列对应肽段免疫动物而制成。该抗体使用多肽亲和层析纯化而得。GABA（γ-氨基丁酸）是中枢神经系统的抑制性神经递质，主要与三个不同受体相互作用：GABA（A），GABA（B）和GABA（C）受体。离子型GABA（A）和GABA（C)受体是配体门控的离子通道，可以对突出传导产生快速抑制。相比而言，代谢型GABA(B)受体与G蛋白偶联，介导对突出传导的慢速抑制（1）。功能性的GABA（B）受体形成GABA（B）R1和GABA（B）R2异二聚体，其中GABA（B）R1与配体结合，GABA（B）R2主要是G蛋白的结合区域（2）。GABA（B）R1也有两种形式：GABA（B）R1a是一个130kD的蛋白，GABA（B）R1b是一个95kD的蛋白（3）。G蛋白随后抑制腺苷酸环化酶的活动，调节肌醇磷脂水解。GABA（B）受体有突触前和突触后抑制：突触前GABA（B）受体通过降低钙通道的高阈值抑制神经递质的释放，突触后GABA（B）受体通过偶联激活内向整流钾通道而产生抑制。除了突出抑制，GABA（B）受体还参与海马长时程增强，慢波睡眠和肌肉放松（1）。

1. Jones, K.A. et al. (2000) Neuropsychopharmacology 23, S41-9.
2. Duthey, B. et al. (2002) J Biol Chem 277, 3236-41.
3. Kaupmann, K. et al. (1997) Nature 386, 239-46.

原厂资料：

Specificity / Sensitivity

GABA(B)R2 Antibody detects endogenous levels of total GABA(B)R2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human GABA(B)R2. Antibodies are purified by peptide affinity chromatography.

Background

GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).

1. Jones, K.A. et al. (2000) Neuropsychopharmacology 23, S41-9.
2. Duthey, B. et al. (2002) J Biol Chem 277, 3236-41.
3. Kaupmann, K. et al. (1997) Nature 386, 239-46.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.