eIF6 (D16E9) XP? Rabbit mAb

• 产品编号：CST-3833S      品牌：CST       原厂货号：3833S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

eIF6 (D16E9) XP® Rabbit mAb检测内源性eIF6总蛋白水平。通过人工合成人源eIF6蛋白相应序列的多肽片段去免疫动物从而制备出单克隆抗体。核仁里60S核糖体亚基的装配需要真核生物起始因子6 (eIF6)(1)。在细胞质中eIF6可以结合到60S核糖体亚基从而阻止它们结合40S核糖体亚基以形成80S核糖体(2)。研究也显示eIF6蛋白与RNA诱导的沉默复合物 (RISC)相互联系(3)。eIF6基因沉默能终止miRNA介导的沉默(3)。eIF6可能通过阻止翻译起始或核糖体循环在miRNA介导的沉默中起到重要的作用(3)。

1. Sanvito, F. et al. (1999) J Cell Biol 144, 823-37.
2. Ceci, M. et al. (2003) Nature 426, 579-84.
3. Chendrimada, T.P. et al. (2007) Nature 447, 823-8.

原厂资料：

Specificity / Sensitivity

eIF6 (D16E9) XP® Rabbit mAb detects endogenous levels of total eIF6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human eIF6.

Background

Eukaryotic initiation factor 6 (eIF6) is reqiured for the 60S ribosomal subunit assembly in the nucleolus (1). In the cytoplasm, this protein is bound to 60S ribosome subunits and prevents them from joining 40S ribosome subunits to form 80S ribosomes (2). eIF6 is also shown to associate with the RNA-induced silencing complex (RISC) (3). Deletion of eIF6 abolishes the miRNA-mediated gene silencing (3). eIF6 may play its essential role in miRNA-mediated silencing by inhibiting translation initiation or ribosome recycling (3).

1. Sanvito, F. et al. (1999) J Cell Biol 144, 823-37.
2. Ceci, M. et al. (2003) Nature 426, 579-84.
3. Chendrimada, T.P. et al. (2007) Nature 447, 823-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.