Phospho-APP (Thr668) Antibody

• 产品编号：CST-3823S      品牌：CST       原厂货号：3823S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-APP (Thr668) Antibody 兔多抗识别内源性β淀粉样蛋白（A4）的前体蛋白的不同亚型，但只有在Thr668（或对其他亚型的相应位置）被磷酸化时才能识别。该多克隆抗体由合成的人类APP695中Thr668位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。淀粉样蛋白β（A4）前体蛋白（APP）是100-140 kDa的跨膜糖蛋白，存在几个亚型（1）。APP的氨基酸序列中包含的淀粉样蛋白域（A-β），它可以通过两步的蛋白裂解反应释放出来（1）。释出的A-β片段在细胞外沉积积累，形成在阿尔茨海默病中淀粉样斑块的主要组成部分（1）。APP可以在多个位点被磷酸化，进而影响其蛋白裂解和分泌过程。（2-5）细胞周期蛋白依赖性激酶介导的Thr688（对应于APP695亚型）磷酸化是细胞周期依赖性的，在G2/M期达到峰值（4）。APP的Thr668磷酸化形式存在于成年鼠大脑和相应的培养神经细胞及其分化细胞中（5,6）。

1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
2. Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.
3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.
4. Suzuki, T. et al. (1994) EMBO J 13, 1114-22.
5. Ando, K. et al. (1999) J Neurosci 19, 4421-7.
6. Iijima, K. et al. (2000) J Neurochem 75, 1085-91.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-APP (Thr668) Antibody detects different isoforms of endogenous amyloid β (A4) precursor protein only when phosphorylated at Thr668 (or the corresponding position on other isoforms).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr668 of human APP695. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Amyloid β (A4) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain (A-β), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released A-β fragments form the main components of amyloid plaques in Alzheimer disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). The phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle dependent and peaks during G2/M-phase (4). The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).

1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
2. Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.
3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.
4. Suzuki, T. et al. (1994) EMBO J 13, 1114-22.
5. Ando, K. et al. (1999) J Neurosci 19, 4421-7.
6. Iijima, K. et al. (2000) J Neurochem 75, 1085-91.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.