GFAT1 Antibody

• 产品编号：CST-3818S      品牌：CST       原厂货号：3818S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GFAT1 Antibody用于检测内源性GFAT1总蛋白水平。多克隆抗体通过用对应于人源GFAT1蛋白序列的合成肽免疫动物制备而得，抗体经蛋白A和肽段亲和层析纯化。谷氨酰胺：6-磷酸果糖氨基转移酶1（GFAT1）是已糖胺合成过程中的限速酶（1）。该酶负责将6－磷酸和谷氨酰胺催化成6－磷酸葡萄糖胺和谷氨酸（2）。已糖胺的生物合成是蛋白质和脂质糖基化的基本途径（2）。除此之外，研究表明，该途径的激活是高糖诱导的胰岛素抵抗中的一个作用因素（1,2）。GFAT1在非胰岛素依赖型的糖尿病病人中活性升高（3）。在脂肪和骨骼肌组织中过表达该酶的转基因小鼠表现出胰岛素抵抗的表型（4,5）。GFAT2是GFAT1的同工酶，随后被发现（6,7）。研究表明GFAT2的调控与GFAT1的不同，这预示着在不同组织中的已糖胺的生物合成途径有着不同的调控方式（7）。

1. Niimi, M. et al. (2001) J Hum Genet 46, 566-71.
2. DeHaven, J.E. et al. (2001) Diabetes 50, 2419-24.
3. Yki-Järvinen, H. et al. (1999) Life Sci 65, 215-23.
4. Cooksey, R.C. et al. (1999) Endocrinology 140, 1151-7.
5. Hebert, L.F. et al. (1996) J Clin Invest 98, 930-6.
6. Oki, T. et al. (1999) Genomics 57, 227-34.
7. Hu, Y. et al. (2004) J Biol Chem 279, 29988-93.

原厂资料：

Specificity / Sensitivity

GFAT1 Antibody detects endogenous levels of total GFAT1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GFAT1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

GFAT1, glutamine:fructose-6-phosphate aminotransferase 1, is the rate-limiting enzyme of the hexosamine biosynthesis pathway (1). This enzyme catalyzes the conversion of fructose-6-phosphate and glutamine to glucosamine-6-phosphate and glutamate (2). The hexosamine biosynthesis pathway generates the building blocks for protein and lipid glycosylation (2). Furthermore, studies suggest that increased activity of this pathway is a contributing factor to hyperglycemia-induced insulin resistance (1,2). GFAT1 is more active in non-insulin-dependent diabetes mellitus (NIDDM) patients (3). Transgenice mice overexpressing this enzyme in skeletal muscle and adipose tissue show an insulin resistance phenotype (4,5). GFAT2, an isoenzyme of GFAT1, was later identified (6, 7). Studies show that the regulation of GFAT2 is different from that of GFAT1, suggesting differential regulation of the hexosamine pathway in different tissues (7).

1. Niimi, M. et al. (2001) J Hum Genet 46, 566-71.
2. DeHaven, J.E. et al. (2001) Diabetes 50, 2419-24.
3. Yki-Järvinen, H. et al. (1999) Life Sci 65, 215-23.
4. Cooksey, R.C. et al. (1999) Endocrinology 140, 1151-7.
5. Hebert, L.F. et al. (1996) J Clin Invest 98, 930-6.
6. Oki, T. et al. (1999) Genomics 57, 227-34.
7. Hu, Y. et al. (2004) J Biol Chem 279, 29988-93.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody