Phospho-PPIG (Ser376) Antibody

• 产品编号：CST-3804S      品牌：CST       原厂货号：3804S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-PPIG (Ser376) Antibody检测仅在Ser376位点磷酸化的内源性PPIG蛋白。通过人工合成人源PPIG蛋白Ser376位点周围相应的磷酸化片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。PPIG是属于高度保守性的亲环蛋白蛋白家族，它最为多肽脯氨酰基异构酶（PPIase）能催化多肽链中反式脯氨酸向顺式脯氨酸转变(1-4)。PPIG蛋白含有一个氨基末端亲环素结构域，其后连接的是Nopp140重复序列，该序列涉及到PPIG的细胞核伴侣功能(5)。PPIG羧基末端含有一个SR (精氨酸丝氨酸双肽重复) 结构域 (3,4），该区域涉及pre-mRNA剪切和加工(6)。PPIG与RNA聚合酶II的羧基末端区域相互作用，也和其它SR家族的剪接因子发生类似的作用。这些相互作用导致PPIG蛋白在定位和构造上的改变，并且提示其在延伸中的RNA聚合酶复合物里转录和pre-mRNA剪接过程中的作用 (7,8)。PPIG蛋白在细胞核基质和核斑点中被发现，其涉及到基因表达的调控。PPIG蛋白在有丝分裂前期主要是在细胞质中弥散分布，并且在有丝分裂后期该异构酶被招募到新形成的细胞核里(9)。Cell Signaling Technology (CST)使用PhosphoScan®（一个CST的 LC-MS/MS 平台）来发现磷酸化位点，PPIG蛋白的Ser376位点的磷酸化被鉴定为ACG激酶的一致性位点(10)。

1. Fischer, G. et al. (1989) Nature 337, 476-8.
2. Freskgård, P.O. et al. (1992) Science 258, 466-8.
3. Nestel, F.P. et al. (1996) Gene 180, 151-5.
4. Mortillaro, M.J. and Berezney, R. (1998) J Biol Chem 273, 8183-92.
5. Meier, U.T. and Blobel, G. (1992) Cell 70, 127-38.
6. Zahler, A.M. et al. (1993) Science 260, 219-22.
7. Lin, C.L. et al. (2004) Biochem Biophys Res Commun 321, 638-47.
8. Bourquin, J.P. et al. (1997) Nucleic Acids Res 25, 2055-61.
9. Dubourg, B. et al. (2004) J Biol Chem 279, 22322-30.
10. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

原厂资料：

Specificity / Sensitivity

Phospho-PPIG (Ser376) Antibody detects endogenous levels of PPIG protein only when phosphorylated at Ser376.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser376 of human PPIG. Antibodies are purified by peptide affinity chromatography.

Background

PPIG belongs to a highly conserved class of cyclophilins that function as peptidyl-prolyl-isomerases (PPIases) to catalyze the conversion of cis-proline to trans-proline in a polypeptide chain (1-4). PPIG contains an amino-terminal cyclophilin domain followed by Nopp140 repeats that are involved in its function as a nuclear chaperone (5). The carboxy-terminal of PPIG contains a SR (arginine-serine dipeptide repeat) domain (3,4) that is involved in pre-mRNA splicing and processing (6). PPIG interacts with the carboxy-terminal domain of RNA polymerase II as well as several other SR family splicing factors. These interactions lead to changes in localization and conformation and suggest a regulatory role in transcription and pre-mRNA splicing in the elongating RNA polymerase complex (7,8). PPIG is found in the nuclear matrix and nuclear speckles and is involved in the regulation of gene expression. PPIG shows a predominantly diffuse cytoplasmic distribution at the onset of mitosis, and in late telophase the isomerase is recruited to the newly formed nuclei (9).

Phosphorylation of Ser376 on PPIG was identified as consensus site fit for ACG kinase at Cell Signaling Technology (CST) using PhosphoScan®, a CST's LC-MS/MS platform for phosphorylation site discovery (10).

1. Fischer, G. et al. (1989) Nature 337, 476-8.
2. Freskgård, P.O. et al. (1992) Science 258, 466-8.
3. Nestel, F.P. et al. (1996) Gene 180, 151-5.
4. Mortillaro, M.J. and Berezney, R. (1998) J Biol Chem 273, 8183-92.
5. Meier, U.T. and Blobel, G. (1992) Cell 70, 127-38.
6. Zahler, A.M. et al. (1993) Science 260, 219-22.
7. Lin, C.L. et al. (2004) Biochem Biophys Res Commun 321, 638-47.
8. Bourquin, J.P. et al. (1997) Nucleic Acids Res 25, 2055-61.
9. Dubourg, B. et al. (2004) J Biol Chem 279, 22322-30.
10. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.