MMP-7 (D4H5) XP? Rabbit mAb

• 产品编号：CST-3801P      品牌：Cell Signaling Technology       原厂货号：3801P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MMP-7 (D4H5) XP®兔源单抗检测内源性总的MMP-7 蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段对应MMP7蛋白的264异亮氨酸附近残基。基质金属蛋白酶（MMP）是一个家族的蛋白酶，靶标多种胞外蛋白，包括其他蛋白酶，生长因子，细胞表面受体和粘附分子（1），在这些家族成员中，MMP-2, MMP-3, MMP-7和 MMP-9在胚胎发育、伤口恢复、肿瘤侵袭、血管生成、致癌和凋亡中已经被认为作为重要因子带有对正常组织重塑（2-4）。MMP活性与癌症发展成正相关（2）。MMP调控的一种机制是转录调控（5）。一旦合成，MMP以潜在的酶原形式存在，MMP活性最大化需要溶蛋白性裂解，通过从全长蛋白释放抑制性前肽结构域而产生活性MMPs（5）。

1. McCawley, L.J. and Matrisian, L.M. (2001) Curr Opin Cell Biol 13, 534-40.
2. Coussens, L.M. et al. (2002) Science 295, 2387-92.
3. Sternlicht, M.D. et al. (1999) Cell 98, 137-46.
4. Vu, T.H. et al. (1998) Cell 93, 411-22.
5. Nagase, H. et al. (1990) Biochemistry 29, 5783-9.

原厂资料：

Specificity / Sensitivity

MMP-7 (D4H5) XP® Rabbit mAb detects endogenous levels of total MMP-7 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile264 of mouse MMP-7 protein.

Background

The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).

1. McCawley, L.J. and Matrisian, L.M. (2001) Curr Opin Cell Biol 13, 534-40.
2. Coussens, L.M. et al. (2002) Science 295, 2387-92.
3. Sternlicht, M.D. et al. (1999) Cell 98, 137-46.
4. Vu, T.H. et al. (1998) Cell 93, 411-22.
5. Nagase, H. et al. (1990) Biochemistry 29, 5783-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.