ERp44 (D17A6) XP? Rabbit mAb

• 产品编号：CST-3798P      品牌：Cell Signaling Technology       原厂货号：3798P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rp44 (D17A6) XP®Rabbit mAb识别内源性的全部ERp44蛋白。该单克隆抗体用与人类ERp44蛋白氨基酸序列对应的人工合成肽段免疫动物而制成。分泌蛋白合成后转位到内质网进行翻译后修饰和正确折叠。为了形成其天然构象，很多分泌蛋白需要形成分子间或分子内二硫键（1）。这个过程成为氧化蛋白折叠。二硫键异构酶（PDI）具有两个硫氧还蛋白同源结构域，并催化这些二硫键形成和异构化（2）。其它驻内质网的拥有硫氧还蛋白同源性区域的蛋白，包括内质网常驻蛋白（ERp44），组成了PDI家族（2）。ERp44在内质网应激时被诱导，通过混合二硫键与Ero1-Lα和Ero1-Lβ连接（3）。Erp44被显示介导了Ero1-Lα的内质网定位（4）。

1. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8.
2. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32.
3. Anelli, T. et al. (2002) EMBO J 21, 835-44.
4. Anelli, T. et al. (2003) EMBO J 22, 5015-22.

原厂资料：

Specificity / Sensitivity

ERp44 (D17A6) XP® Rabbit mAb detects endogenous levels of total ERp44 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ERp44.

Background

Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum resident protein 44 (ERp44), constitute the PDI family (2). ERp44 is induced upon ER stress and is linked to Ero1-Lα and Ero1-Lβ through mixed disulfide bonds (3). ERp44 was shown to mediate the ER localization of Ero1-Lα (4).

1. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8.
2. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32.
3. Anelli, T. et al. (2002) EMBO J 21, 835-44.
4. Anelli, T. et al. (2003) EMBO J 22, 5015-22.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.