α-Amylase (D55H10) XP? Rabbit mAb

• 产品编号：CST-3796P      品牌：Cell Signaling Technology       原厂货号：3796P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

α-Amylase (D55H10) XP＠ Rabbit mAb用于检测内源性α-Amylase总蛋白水平。单克隆抗体通过用对应于人源α-Amylase蛋白序列的合成肽免疫动物制备而得。α淀粉酶负责催化寡聚糖和多聚糖4-D-糖苷键的裂解反应（1）。正常情况下该酶仅在唾液腺（唾液α淀粉酶或AMY1）和胰腺（胰腺α淀粉酶或AMY2A）中产生并分泌出来（1）。最近研究发现一些肿瘤细胞会异位表达并分泌α淀粉酶（1）。此外，在肺良性肿瘤中发现了一种新的α淀粉酶（良性瘤α淀粉酶或AMY2B）（2－4）。同时在神经内分泌肿瘤中α淀粉酶的异位表达也有报导（5）。

1. Tomita, N. et al. (1988) Cancer Res 48, 3292-6.
2. Tomita, N. et al. (1989) Gene 76, 11-8.
3. Groot, P.C. et al. (1989) Gene 85, 567-8.
4. Yokouchi, H. et al. (1990) Gene 90, 281-6.
5. Daa, T. et al. (2002) J Exp Clin Cancer Res 21, 131-6.

原厂资料：

Specificity / Sensitivity

α-Amylase (D55H10) XP® Rabbit mAb detects endogenous levels of total α-amylase protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human α-amylase.

Background

α-amylase catalyzes the cleavage of 1, 4-α-D-glucosidic bonds in oligosaccharides and polysaccharides (1). The enzyme is normally produced and secreted in salivary glands (salivary α-amylase or AMY1) and pancreas (pancreatic α-amylase or AMY2A) (1). Studies reported the release of an ectopically expressed α-amylase in certain tumors (1). Furthermore, a new type of α-amylase (carcinoid α-amylase or AMY2B) was identified in a lung carcinoid tissue (2-4). The ectopic expression of α-amylase in a neuroendocrine tumor was also reported (5).

1. Tomita, N. et al. (1988) Cancer Res 48, 3292-6.
2. Tomita, N. et al. (1989) Gene 76, 11-8.
3. Groot, P.C. et al. (1989) Gene 85, 567-8.
4. Yokouchi, H. et al. (1990) Gene 90, 281-6.
5. Daa, T. et al. (2002) J Exp Clin Cancer Res 21, 131-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.