Phospho-HER4/ErbB4 (Tyr984) Antibody

• 产品编号：CST-3790S      品牌：CST       原厂货号：3790S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化HER4/ErbB4 (Tyr984) 抗体仅在Tyr984位点磷酸化时能检测到内源HER4/ErbB4蛋白的表达。本抗体可能与其它酪氨酸磷酸化的蛋白发生交叉反应。多克隆抗体通过用多肽免疫动物得到，该磷酸化多肽是根据人的HER4/ErbB4蛋白Tyr984附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。研究表明HER/ErbB酪氨酸激酶受体家族在正常发育、心脏功能和肿瘤中发挥作用（1,2）。HER4/ErbB4和家族其它成员一样，有四个胞外功能区，一个跨膜区和一个具有激活酪氨酸激酶活性的胞内区（3）。通过结合神经调节素(NRGs)和/或EGF家族配体，ErbB4形成同源二聚体或与ErbB家族的其它成员形成异二聚体，进而使受体活化进行信号传递（3）。ErbB4普遍高表达于脑和心脏。ErbB4在乳腺癌、小儿脑癌和其它种类肿瘤上的表达和正常组织发育以及肿瘤的发生均有关系（3）。

1. Holbro, T. and Hynes, N.E. (2004) Annu Rev Pharmacol Toxicol 44, 195-217.
2. Roskoski, R. (2004) Biochem Biophys Res Commun 319, 1-11.
3. Carpenter, G. (2003) Exp Cell Res 284, 66-77.

原厂资料：

Specificity / Sensitivity

Phospho-HER4/ErbB4 (Tyr984) Antibody detects endogenous levels of HER4/ErbB4 protein only when phosphorylated on Tyr984. This antibody may cross-react with other tyrosine-phosphorylated proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr984 of human HER4/ErbB4. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The HER/ErbB receptor tyrosine kinase family has been implicated in normal development, cardiac function and cancer (1,2). HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain and a cytoplasmic tail containing the active tyrosine kinase domain (3). By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling (3). ErbB4 is ubiquitously expressed with the highest expression occurring in brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer and other types of carcinomas has been reported suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis (3).

1. Holbro, T. and Hynes, N.E. (2004) Annu Rev Pharmacol Toxicol 44, 195-217.
2. Roskoski, R. (2004) Biochem Biophys Res Commun 319, 1-11.
3. Carpenter, G. (2003) Exp Cell Res 284, 66-77.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.