Phospho-Jak2 (Tyr1007/1008) Antibody

• 产品编号：CST-3771S      品牌：CST       原厂货号：3771S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Jak2 (Tyr1007/1008) 抗体只能检测内源的在Tyr1007/1008位点磷酸化的Jak2蛋白。抗体也可与磷酸化的Jak1 和 Tyk2蛋白交叉反应。此多克隆抗体是通过合成人源对应的Jak2 Tyr1007/1008位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。Janus家族中的酪氨酸激酶成员(Jak1, Jak2, Jak3和Tyk2)通过配体结合到一些相关的细胞因子受体上而被激活（1）。除了细胞因子受体的激活方式，Jak蛋白能自身磷酸化和磷酸化与其相关的受体从而为信号蛋白提供多个结合位点。这些信号蛋白如Stats (2), Shc (3), 胰岛素受体底物(4) and 成簇黏附激酶 (FAK) (5)都是典型的含有SH2 或者其它的磷酸化酪氨酸结合区域。Jak2酪氨酸残基1022/1023存在于可能的活性环中，与Tyk2中的酪氨酸残基1054/1055属于同源残基，这两个残基对Tyk2激酶活性的调节有重要的作用(6)。

1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
2. Darnell, J.E. (1997) Science 277, 1630-5.
3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
6. Gauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-20500.

原厂资料：

Specificity / Sensitivity

Phospho-Jak2 (Tyr1007/1008) Antibody detects endogenous levels of Jak2 only when phosphorylated at Tyr1007/1008. This antibody may cross-react with phospho-Jak1 and phospho-Tyk2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1007/1008 of human Jak2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

The tyrosine residues 1007/1008 of Jak2 in the putative activation loop are the homologous tyrosine residues 1054/1055 in Tyk2, which are important in the regulation of Tyk2 kinase activity (6).

1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
2. Darnell, J.E. (1997) Science 277, 1630-5.
3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
6. Gauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-20500.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.