BNIP3 Antibody (Rodent Specific)

• 产品编号：CST-3769S      品牌：CST       原厂货号：3769S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BNIP3 Antibody (Rodent Specific) 能够检测内源性的小鼠和大鼠的BNIP3总蛋白。该多克隆抗体是由合成的小鼠/大鼠源BNIP3蛋白 Ser60位周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。BNIP3（Bcl-2/E1B-19kDa互作蛋白3）是一种促凋亡线粒体蛋白，是Bcl-2家族成员之一，包含一个Bcl-2同源3（BH3）结构域和羧基末端跨膜（TM）域（1-3）。预测BNIP3分子量约22kDa ，然而在SDS-PAGE的结果中还出现了一个约60 kDa的条带，这可能是其尚未分解的二聚体（2）。BNIP3与抗凋亡的Bcl-2家族成员Bcl-xL和腺病毒同源物E1B-19kDa协同作用。不同于其他只含有BH3结构域的Bcl-2家族成员， BNIP3还有一个TM域，TM域对于其线粒体定位和促凋亡活性是必需的，而不是BH3结构域（4）。除了细胞凋亡，BNIP3还与坏死（5）和自噬（6,7）相关，但是BNIP3和这些过程之间的关系尚不完全清楚。在缺氧条件下，BNIP3表达增加，这主要受转录因子HIF-1α的调控（8-10）。人们已经在几种类型的癌症细胞中，观察到BNIP3启动子的甲基化沉默，这可能在它们的生存中可能起着重要的作用（11-14）。

1. Boyd, J.M. et al. (1994) Cell 79, 341-51.
2. Chen, G. et al. (1997) J Exp Med 186, 1975-83.
3. Yasuda, M. et al. (1998) J Biol Chem 273, 12415-21.
4. Ray, R. et al. (2000) J Biol Chem 275, 1439-48.
5. Vande Velde, C. et al. (2000) Mol Cell Biol 20, 5454-68.
6. Daido, S. et al. (2004) Cancer Res 64, 4286-93.
7. Tracy, K. et al. (2007) Mol Cell Biol 27, 6229-42.
8. Quinsay, M.N. et al. (2010) Autophagy 6, 855-62.
9. Bellot, G. et al. (2009) Mol Cell Biol 29, 2570-81.
10. Lee, Y. et al. (2011) Am J Physiol Heart Circ Physiol 301, H1924-31.
11. Hanna, R.A. et al. (2012) J Biol Chem 287, 19094-104.
12. Bruick, R.K. (2000) Proc Natl Acad Sci USA 97, 9082-7.
13. Guo, K. et al. (2001) Cell Death Differ 8, 367-76.
14. Sowter, H.M. et al. (2001) Cancer Res 61, 6669-73.
15. de Angelis, P.M. et al. (2004) Int J Oncol 24, 1279-88.
16. Okami, J. et al. (2004) Cancer Res 64, 5338-46.
17. Murai, M. et al. (2005) Clin Cancer Res 11, 1021-7.
18. Murai, M. et al. (2005) Br J Cancer 92, 1165-72.

原厂资料：

Specificity / Sensitivity

BNIP3 Antibody (Rodent Specific) detects endogenous levels of total BNIP3 protein from mouse and rat.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser60 of BNIP3 from mouse/rat. Antibodies were purified by peptide affinity chromatography.

Background

BNIP3 (Bcl-2/E1B-19kDa interacting protein 3) is a pro-apoptotic mitochondrial protein and Bcl-2 family member that contains a Bcl-2 homology 3 (BH3) domain and a carboxyl terminal transmembrane (TM) domain (1-3). While BNIP3 has a predicted molecular weight of about 22 kDa, it runs anomalously on SDS-PAGE and includes a band of around 60 kDa that may be a dimeric form that is not reduced (2). BNIP3 associates with anti-apoptotic family members Bcl-2, Bcl-xL, and the adenovirus homologue E1B-19kDa. BNIP3 is distinct from other Bcl-2 family members that contain only the BH3 domain in that the TM domain, and not the BH3 domain, is required for mitochondrial targeting and pro-apoptotic activity (4). In addition to apoptosis, BNIP3 has been implicated in necrosis (5) and autophagy (6,7), although the relationship between BNIP3 and these processes is not fully understood. Increased expression of BNIP3 under hypoxic conditions is mainly regulated by the transcription factor HIF-1α (8-10). Silencing of the BNIP3 promoter by methylation has been observed in several types of cancer cells and may play an important role in their survival (11-14).

1. Boyd, J.M. et al. (1994) Cell 79, 341-51.
2. Chen, G. et al. (1997) J Exp Med 186, 1975-83.
3. Yasuda, M. et al. (1998) J Biol Chem 273, 12415-21.
4. Ray, R. et al. (2000) J Biol Chem 275, 1439-48.
5. Vande Velde, C. et al. (2000) Mol Cell Biol 20, 5454-68.
6. Daido, S. et al. (2004) Cancer Res 64, 4286-93.
7. Tracy, K. et al. (2007) Mol Cell Biol 27, 6229-42.
8. Quinsay, M.N. et al. (2010) Autophagy 6, 855-62.
9. Bellot, G. et al. (2009) Mol Cell Biol 29, 2570-81.
10. Lee, Y. et al. (2011) Am J Physiol Heart Circ Physiol 301, H1924-31.
11. Hanna, R.A. et al. (2012) J Biol Chem 287, 19094-104.
12. Bruick, R.K. (2000) Proc Natl Acad Sci USA 97, 9082-7.
13. Guo, K. et al. (2001) Cell Death Differ 8, 367-76.
14. Sowter, H.M. et al. (2001) Cancer Res 61, 6669-73.
15. de Angelis, P.M. et al. (2004) Int J Oncol 24, 1279-88.
16. Okami, J. et al. (2004) Cancer Res 64, 5338-46.
17. Murai, M. et al. (2005) Clin Cancer Res 11, 1021-7.
18. Murai, M. et al. (2005) Br J Cancer 92, 1165-72.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.