Phospho-IP3 Receptor (Ser1756) Antibody

• 产品编号：CST-3760S      品牌：CST       原厂货号：3760S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-IP3 Receptor(Ser1756)抗体识别内源性的Ser1756磷酸化的IP3受体。该多克隆抗体用与大鼠IP3受体中Ser1756位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物而制成。该抗体使用蛋白亲和层析纯化而得。肌醇1,4,5 - 三磷酸受体，也被称为IP3R 或InsP3R，是细胞内钙释放通道家族的成员，位于内质网中。IP3R可作为Ca2 +细胞内储存的钙离子释放通道的功能。有三种IP3受体类型（IP3R1，2，和3），第二信使激活肌醇1,4,5 - 三磷酸（IP3）（1）。四个独立的亚基同源或异源寡聚体化以形成受体的功能通道（2）。IP3R1丝氨酸（1756位）磷酸化，通过环腺苷酸AMP依赖性蛋白激酶（PKA）调节IP3R1至IP3的灵敏性，可能作为Ca2 +释放调节模型(3,4)。IP3R1介导的Ca2 +释放似乎对蒲金耶氏细胞长时程抑制（LTD）的诱导有影响（5）。

1. Joseph, S.K. (1996) Cell Signal 8, 1-7.
2. Galvan, D.L. et al. (1999) J Biol Chem 274, 29483-92.
3. Haug, L.S. et al. (1999) J Biol Chem 274, 7467-73.
4. DeSouza, N. et al. (2002) J Biol Chem 277, 39397-400.
5. Inoue, T. et al. (1998) J Neurosci 18, 5366-73.

原厂资料：

Specificity / Sensitivity

Phospho-IP3 Receptor (Ser1756) Antibody detects endogenous levels of IP3 Receptor only when phosphorylated at Ser1756.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1756 of rat IP3 receptor. Antibodies are purified by peptide affinity chromatography.

Background

Inositol 1,4,5-triphosphate receptor, also known as IP3R or InsP3R, is a member of the intracellular calcium release channel family and is located in the endoplasmic reticulum. It functions as a Ca2+ release channel for intracellular stores of calcium ions. There are three types of IP3 receptors (IP3R1, 2 and 3) that require the second messenger inositol 1,4,5-triphosphate (IP3) for activation (1). Four individual receptor subunits, resulting in homo- or hetero-oligomerization of the receptor isoform, form a functional channel (2). Phosphorylation of IP3R1 at Ser1756 by cyclic-AMP-dependent protein kinase A (PKA) regulates the sensitivity of IP3R1 to IP3 and may thus be a mode of regulation for Ca2+ release (3,4). IP3R1-mediated Ca2+ release appears to have an effect on the induction of long term depression (LTD) in Purkinje cells (5).

1. Joseph, S.K. (1996) Cell Signal 8, 1-7.
2. Galvan, D.L. et al. (1999) J Biol Chem 274, 29483-92.
3. Haug, L.S. et al. (1999) J Biol Chem 274, 7467-73.
4. DeSouza, N. et al. (2002) J Biol Chem 277, 39397-400.
5. Inoue, T. et al. (1998) J Neurosci 18, 5366-73.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.