SHP-1 (C14H6) Rabbit mAb

• 产品编号：CST-3759S      品牌：CST       原厂货号：3759S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SHP-1 (C14H6) Rabbit mAb能够检测内源SHP-1蛋白总体水平。单克隆抗体是通过合成人源对应的SHP-1 Pro161位点周围的肽段来免疫动物而获得。SHP-1 (PTPN6)是一个无受体蛋白的酪氨酸磷酸酶，主要在造血细胞中表达。这个酶有两个SH2区域、一个酪氨酸磷酸酶催化区域和C-末端调控区域组成(1)。SHP-1能够从目标蛋白上去除磷酸根从而下调几个酪氨酸激酶调控通路。在造血细胞中, SHP-1蛋白的N-末端SH2区域结合到酪氨酸磷酸化的红细胞生成素受体(EpoR)，并负调控造血组织的生长(2)。在上皮细胞中过表达SHP-1导致Ros受体的酪氨酸激酶的去磷酸化并随后下调Ros依赖的细胞增殖和转化(3)。在骨髓细胞中配体结合后, SHP-1 与 IL-3R β链作用并下调IL-3诱导的酪氨酸磷酸化和细胞增殖(4)。因为SHP-1下调很多增殖的信号通路, SHP-1被看做是潜在的肿瘤抑制因子和血管再生调控因子(5,6)。

1. Yi, T.L. et al. (1992) Mol Cell Biol 12, 836-46.
2. Yi, T. et al. (1995) Blood 85, 87-95.
3. Keilhack, H. et al. (2001) J Cell Biol 152, 325-34.
4. Yi, T. et al. (1993) Mol Cell Biol 13, 7577-86.
5. Wu, C. et al. (2003) Gene 306, 1-12.
6. Bhattacharya, R. et al. (2008) J Mol Signal 3, 8.

原厂资料：

Specificity / Sensitivity

SHP-1 (C14H6) Rabbit mAb detects endogenous level of total SHP-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro161 of human SHP-1.

Background

SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain and a carboxy-terminal regulatory domain (1). SHP-1 removes phosphates from target proteins to down regulate several tyrosine kinase regulated pathways. In hematopoietic cells, the N-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth (2). Over expression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation (3). Following ligand binding in myeloid cells, SHP-1 associates with IL-3R β chain and down regulates IL-3-induced tyrosine phosphorylation and cell proliferation (4). Because SHP-1 down regulates various proliferation pathways, SHP-1 is considered a potential tumor suppressor and angiogenesis regulator (5,6).

1. Yi, T.L. et al. (1992) Mol Cell Biol 12, 836-46.
2. Yi, T. et al. (1995) Blood 85, 87-95.
3. Keilhack, H. et al. (2001) J Cell Biol 152, 325-34.
4. Yi, T. et al. (1993) Mol Cell Biol 13, 7577-86.
5. Wu, C. et al. (2003) Gene 306, 1-12.
6. Bhattacharya, R. et al. (2008) J Mol Signal 3, 8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.