SNIP/p140Cap Antibody

• 产品编号：CST-3757S      品牌：CST       原厂货号：3757S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SNIP/p140Cap Antibody识别内源性的SNIP/p140Cap总蛋白。该多克隆抗体用与人类SNIP/p140Cap蛋白羧基末端附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和肽亲和层析纯化而得。SNIP (SNAP25互作蛋白)/p140Cap (p130Cas相关蛋白)是细胞骨架相关蛋白，最初在大鼠中发现，是一种可以与脑特异性突触体蛋白SNAP25(1)相互作用的蛋白，后来发现它可以和广泛表达的折叠蛋白质p130Cas(2)相互作用。SNAP25是一个突触前蛋白，可能与神经递质分泌、膜融合、神经突生长相关，是SNARE复合物的一部分，包含syntaxin和snaptobrevin/VAMP（3）。SNIP-SNAP25的结合由卷曲螺旋相互作用介导（1）。过表达SNIP抑制PC12细胞的钙依赖的胞吐（1）。人和鼠SNIP同源直系物，称为p140Cap，通过与一种v-Src和v-Crk的底物p130Cas结合而被发现，该物质在细胞黏附和促有丝分裂刺激下发生酪氨酸磷酸化（2,4,5）。p140Cap在脑、睾丸和富含上皮的组织中表达，并且可能以多个可变剪切异构体特异性地在不同组织中存在（2）。p140Cap在响应黏附分子和EGF处理时酪氨酸被磷酸化（2）。综合这些研究显示SNIP/p140Cap在控制细胞扩展、迁移和神经分泌中扮演重要作用。

1. Chin, L.S. et al. (2000) J. Biol. Chem. 275, 1191-1200.
2. Di Stefano, P. et al. (2004) Mol. Biol. Cell 15, 787-800.
3. Gerst, J.E. (1999) Cell. Mol. Life Sci. 55, 707-734.
4. Sakai, R. et al. (1994) EMBO J. 13, 3748-3756.
5. Defilippi, P. et al. (2006) Trends Cell Biol. 16, 257-263.

原厂资料：

Specificity / Sensitivity

SNIP/p140Cap Antibody detects endogenous levels of total SNIP/p140Cap protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SNIP/p140Cap. Antibodies were purified by protein A and peptide affinity chromatography.

Background

SNIP (SNAP25-interacting protein)/p140Cap (p130Cas-associated protein) is a cytoskeleton-associated protein identified initially in rat as a protein interacting with the brain-specific synaptosome protein SNAP25 (1) and subsequently as interacting with the broadly expressed scaffold protein p130Cas (2). SNAP25, a presynaptic protein implicated in neurotransmitter secretion, membrane fusion and neurite outgrowth, is part of the SNARE complex that includes syntaxin and synaptobrevin/VAMP (3). SNIP-SNAP25 association is mediated by coiled-coil interactions (1). Overexpression of SNIP inhibits calcium-dependent exocytosis in PC12 cells (1). Human and mouse orthologs of SNIP, termed p140Cap, were subsequently identified through association with p130Cas, a substrate of v-Src and v-Crk that is tyrosine-phosphorylated in response to cell adhesion and mitogenic stimuli (2,4,5). Expression of p140Cap was observed in brain, testis and epithelial-rich tissues and may exist in various alternatively spliced, tissue-specific isoforms (2). p140Cap is also tyrosine-phosphoryalated in response to adhesion molecules and EGF treatment (2). Together these studies suggest a role for SNIP/p140Cap in controlling cell spreading, migration and neurosecretion.

1. Chin, L.S. et al. (2000) J. Biol. Chem. 275, 1191-1200.
2. Di Stefano, P. et al. (2004) Mol. Biol. Cell 15, 787-800.
3. Gerst, J.E. (1999) Cell. Mol. Life Sci. 55, 707-734.
4. Sakai, R. et al. (1994) EMBO J. 13, 3748-3756.
5. Defilippi, P. et al. (2006) Trends Cell Biol. 16, 257-263.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.