TNF-R1 (C25C1) Rabbit mAb

• 产品编号：CST-3736S      品牌：CST       原厂货号：3736S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TNF-R1 (C25C1) Rabbit mAb兔单抗能够检测内源TNF-R1蛋白总体水平。此蛋白不与其它相关家族的蛋白交叉反应。此单克隆抗体是通过合成人源对应的TNF-R1 细胞内Ser331位点周围的肽段来免疫动物而获得。TNF-α是一个重要的细胞因子，它可由很多种类的细胞产生包括中性粒细胞，激活的淋巴细胞，巨噬细胞和NK细胞。它在炎症反应和凋亡过程中发挥了重要的作用(1)。TNF-α存在细胞膜锚定和可溶性形式，这两种形式都有生物活性。对TNF-α的响应是通过两个受体介导的，一个是广泛表达的TNF-R1，另一个是主要在免疫和内皮细胞表达的TNF-R2(2)。 TNF-α的拮抗剂已经被确定为治疗风湿性关节炎和免疫紊乱的靶标(3)。TNF-α的两个受体, TNF-R1 (55 kDa) 和 TNF-R2 (75 kDa) 能够介导不同的细胞应答(4,5)。据报道很多时候TNF引导的细胞毒性是通过TNF-R1(6,7)。细胞毒性是通过一个细胞内的死亡区域受体而介导的，然后结合到接头蛋白TRADD上并引起信号通路的激活(8)。这两个可溶性的受体都已经被鉴定，他们能够结合TNF-α并在免疫紊乱过程中发挥了重要的作用(9,10)。

1. Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56.
2. Locksley, R.M. et al. (2001) Cell 104, 487-501.
3. Taylor, P.C. et al. (2004) Curr Opin Biotechnol 15, 557-63.
4. Tartaglia, L.A. et al. (1991) Proc Natl Acad Sci USA 88, 9292-6.
5. Peschon, J.J. et al. (1998) J Immunol 160, 943-52.
6. Tartaglia, L.A. et al. (1993) Cell 73, 213-6.
7. Rothe, J. et al. (1993) Nature 364, 798-802.
8. Chen, G. and Goeddel, D.V. (2002) Science 296, 1634-5.
9. Humbert, M. et al. (1994) Am J Respir Crit Care Med 149, 1681-5.
10. Schröder, J. et al. Infection 23, 143-8.

原厂资料：

Specificity / Sensitivity

TNF-R1 (C25C1) Rabbit mAb detects endogenous levels of human TNF-R1 protein. This antibody does not appear to cross-react with other related family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues within the intracellular region and surrounding Ser331 of human TNF-R1 protein.

Background

TNF-α is an important cytokine produced by numerous cell types including neutrophils, activated lymphoctyes, macrophages an NK cells. It plays a critical role in inflammatory responses and in apoptosis (1). TNF-α exists as a membrane-anchored and soluble form, both of which show biological activity. Response to TNF-α is mediated through two receptors, TNF-R1, which is widely expressed, and TNF-R2, which is expressed mainly in immune and endothelial cells (2). Antagonists to TNF-α have been validated as therapeutic targets for rheumatoid arthritis and other immune disorders (3).

The two receptors for TNF-α, TNF-R1 (55 kDa) and TNF-R2 (75 kDa) can mediate distinct cellular responses (4,5). In most cases cytotoxicity elicited by TNF has been reported to act through TNF-R1 (6,7). Cytotoxicity is mediated by a "death domain" with the intracellular region of the receptor that binds to the death domain adaptor protein TRADD and triggers the activation of caspases (8). Soluble forms of both receptors have also been characterized which can bind TNF-α and may play an important role in immune disorders (9,10).

1. Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56.
2. Locksley, R.M. et al. (2001) Cell 104, 487-501.
3. Taylor, P.C. et al. (2004) Curr Opin Biotechnol 15, 557-63.
4. Tartaglia, L.A. et al. (1991) Proc Natl Acad Sci USA 88, 9292-6.
5. Peschon, J.J. et al. (1998) J Immunol 160, 943-52.
6. Tartaglia, L.A. et al. (1993) Cell 73, 213-6.
7. Rothe, J. et al. (1993) Nature 364, 798-802.
8. Chen, G. and Goeddel, D.V. (2002) Science 296, 1634-5.
9. Humbert, M. et al. (1994) Am J Respir Crit Care Med 149, 1681-5.
10. Schröder, J. et al. Infection 23, 143-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.