MCM7 (D10A11) XP? Rabbit mAb

• 产品编号：CST-3735P      品牌：Cell Signaling Technology       原厂货号：3735P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MCM7 (D10A11) XP® Rabbit mAb能够检测内源性MCM7总蛋白。Western blot和免疫荧光资料提示该抗体对于灵长类动物蛋白的反应性优于啮齿类动物蛋白。.该单克隆抗体是由合成的人源的针对MCM7蛋白的羧基末端肽段免疫动物生产的。微小染色体维持（MCM）蛋白2-7是DNA复制起始和延伸所需的6个相关蛋白家族。 MCM2到MCM7蛋白相互结合，形成异源六聚体MCM复合物，被认为充当了DNA复制叉的复制解旋酶（1-5）。该复合体也是前复制复合体(pre-RC)的关键组成部分(回顾1)。Cdc6 和 Cdt1招募MCM复合体至有丝分裂末期/G1早期的起始识别复合体(ORC)形成pre-RC，促进DNA复制(2中已论述)。MCM2, MCM3, MCM4和MCM6亚单位的磷酸化似乎调节MCM复合体的活性和DNA合成的启动(6-8)。DNA复制时MCM蛋白被清除，通过pre-RC重构的抑制引起染色质未识别化。染色质批准允许每个细胞循环DNA复制一次，有助于保证基因选择和避免恶性肿瘤细胞的产生(回顾3)。研究亦表明MCM复合体通过保护复制叉的结构参与检验点控制，而且可以招募捕获后的检验点蛋白协助复制重启(回顾3,9)。

1. Lei, M. and Tye, B.K. (2001) J Cell Sci 114, 1447-54.
2. Lygerou, Z. and Nurse, P. (2000) Science 290, 2271-3.
3. Forsburg, S.L. (2004) Microbiol Mol Biol Rev 68, 109-31.
4. Tye, B.K. and Sawyer, S. (2000) J Biol Chem 275, 34833-6.
5. Labib, K. et al. (2000) Science 288, 1643-7.
6. Charych, D.H. et al. (2008) J Cell Biochem 104, 1075-86.
7. Masai, H. et al. (2006) J Biol Chem 281, 39249-61.
8. Lin, D.I. et al. (2008) Proc Natl Acad Sci USA 105, 8079-84.
9. Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72.
10. Bailis, J.M. et al. (2008) Mol Cell Biol 28, 1724-38.

原厂资料：

Specificity / Sensitivity

MCM7 (D10A11) XP® Rabbit mAb detects endogenous levels of total MCM7 protein. Western blot analysis and immunofluorescent data indicate that the antibody is more reactive to primate than rodent proteins.MCM7 (D10A11)

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human MCM7.

Background

The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2 through MCM7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and Cdt1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4 and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have also shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,9).

1. Lei, M. and Tye, B.K. (2001) J Cell Sci 114, 1447-54.
2. Lygerou, Z. and Nurse, P. (2000) Science 290, 2271-3.
3. Forsburg, S.L. (2004) Microbiol Mol Biol Rev 68, 109-31.
4. Tye, B.K. and Sawyer, S. (2000) J Biol Chem 275, 34833-6.
5. Labib, K. et al. (2000) Science 288, 1643-7.
6. Charych, D.H. et al. (2008) J Cell Biochem 104, 1075-86.
7. Masai, H. et al. (2006) J Biol Chem 281, 39249-61.
8. Lin, D.I. et al. (2008) Proc Natl Acad Sci USA 105, 8079-84.
9. Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72.
10. Bailis, J.M. et al. (2008) Mol Cell Biol 28, 1724-38.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.