Neuropilin-1 (D62C6) Rabbit mAb

• 产品编号：CST-3725S      品牌：CST       原厂货号：3725S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Neuropilin-1 (D39A5)兔单克隆抗体识别内源性的全部neuropilin-1蛋白。.该单克隆抗体用与人类neuropilin-1蛋白的氨基酸序列对应的GST融合的人工合成肽段免疫动物而制成。Class 3 secreted semaphorin (Sema3A)是多种轴突上的神经生长导向因子。因此，它可以诱导微丝发生戏剧性的再分布和解聚，导致生长锥崩塌。Plexins是单次跨膜信号转导蛋白，包括Plexin A1，A2，A3和A4。Plexins可以与neuropilin-1、neuropilin-2和细胞黏附蛋白L1形成功能性的semaphorin受体（1,2）。GTP酶Rnd1结合Plexin A1的细胞质结构域，触发细胞骨架崩溃。相反，GTP酶RndD阻止Rnd1-介导的Plexin A1的活性和Sema3A所致交感神经轴突的排斥（3）。).Neuropilin-1是一个单次跨膜的糖蛋白，最初确定为介导的轴突生长锥崩塌的semaphorin受体。此外，neuropilin-1参与轴索成束，神经细胞迁移，树突导向和成人神经系统的修复（4）。Neuropilin-1在肿瘤和内皮细胞上还是一个VEGF受体。Neuropilin-1功能缺失可以导致血管重塑和分支障碍，进一步伴有neuropilin-2功能障碍（4,5）。

1. Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89.
2. Takahashi, T. and Strittmatter, S.M. (2001) Neuron 29, 429-39.
3. Zanata, S.M. et al. (2002) J Neurosci 22, 471-7.
4. He, Z. et al. (2002) Sci STKE 2002, RE1.
5. Kawasaki, T. et al. (1999) Development 126, 4895-902.
6. Takashima, S. et al. (2002) Proc Natl Acad Sci USA 99, 3657-62.

原厂资料：

Specificity / Sensitivity

Neuropilin-1 (D39A5) Rabbit mAb detects endogenous levels of total neuropilin-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST-fusion protein corresponding to residues of mouse neuropilin-1.

Background

Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single pass, transmembrane signaling proteins encompassing Plexin A1, A2, A3 and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).

Neuropilin-1, a single pass transmembrane glycoprotein, was originally identified as a semaphorin receptor mediating axon growth cone collapse. In addition, neuropilin-1 is involved in axonal fasciculation, neuronal migration, dendritic guidance and repair of the adult nervous system (4). Neuropilin-1 is also an isoform-specific VEGF receptor on tumor and endothelial cells. Loss of neuropilin-1 function causes vascular remodeling and branching defects, which is compounded by additional loss of neuropilin-2 function (4, 5).

1. Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89.
2. Takahashi, T. and Strittmatter, S.M. (2001) Neuron 29, 429-39.
3. Zanata, S.M. et al. (2002) J Neurosci 22, 471-7.
4. He, Z. et al. (2002) Sci STKE 2002, RE1.
5. Kawasaki, T. et al. (1999) Development 126, 4895-902.
6. Takashima, S. et al. (2002) Proc Natl Acad Sci USA 99, 3657-62.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.