Pro-TGF-alpha Antibody

• 产品编号：CST-3715S      品牌：CST       原厂货号：3715S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Pro-TGF-alpha 抗体识别内源性的TGF-alpha前体蛋白，而不识别成熟的TGF-alpha蛋白， 它与其他家族成员在生理条件下无交叉反应.多克隆抗体由合成的肽段免疫动物产生，该肽段与人类TGF-alpha蛋白氨基末端的氨基酸序列一致。抗体由肽段由蛋白A和亲和层析技术纯化.转化生长因子alpha (TGF-alpha)是表皮生长因子（EGF）家族成员，与EGFR有共同的受体参与调控了细胞增殖，存活和分化。（1）家族成员享有一个EGF样结构域45-60个氨基酸，以标志的保守的六个半胱氨酸结构，形成三个二硫键，有结合受体结构域功能。TGF-alpha起始在逆转录病毒转化的成纤维细胞培养基中被发现，其名字来自于它能诱导培养中的成纤维细胞转化（2）。转化活性显示需要TGF-beta, 这可通过分离的受体加强TGF-alpha活性（3）。可溶性TGF-alpha从它的膜结合前体前TGF-alpha释放出来，紧随着质子剪切，但膜结合前体仍旧能结合并激活EGFR（4）。可溶性或膜结合TGF-alpha与EGFR结合导致受体二聚化，酪氨酸自磷酸化，下游信号成员的激活。TGF-alpha和相关肽段对癌症和神经病理过程起重要作用（5,6）.

1. Derynck, R. (1986) J. Cell Biochem. 32, 203-204.
2. de Larco, J.E. and Tordaro, G.J. (1978) Proc. Natl. Acad. Sci. USA 75, 4001-4005.
3. Roberts, A. B. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 5339-5343.
4. Wong, S. T. et al. (1989) Cell 56, 495-506.
5. Rusch, V. et al. (1996) Cytokine Growth Factor Rev. 7, 133-141.
6. Junier, M.P. (2000) Prog. Neurobiol. 62, 443-473.

原厂资料：

Specificity / Sensitivity

Pro-TGF-alpha Antibody detects enogenous levels TGF-alpha precusor protein but not mature TGF-alpha. It does not cross-react with other family members at physiological levels.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of TGF-alpha . Antibodies are purified by protein A and peptide affinity chromatography.

Background

Transforming growth factor alpha (TGF-alpha) is a member of the epidermal growth factor (EGF) family, sharing the same receptor, EGFR, and regulating cell proliferation, survival, and differention (1). Members of the family share an EGF-like domain of 45-60 amino acids characterized by the conservation of six regularly spaced cysteines, forming three disulfide bonds that function as their receptor binding domain. TGF-alpha was initially discovered in the media of retrovirally transformed fibroblasts, and it name comes from its ability to induce transformation in cultured fibroblasts (2). This transforming activity was later shown to require TGF-beta, which potentiates the activity of TGF-alpha through a separate receptor (3). Soluble TGF-alpha is released from its membrane-bound precusor, pro-TGF-alpha, following protolytic cleavage, but the membrane bound precursor is still able to bind and activate EGFR (4). Binding of soluble or membrane bound TGF-alpha to EGFR leads to receptor dimerization, tyrosine autophosphorylation, and activation of downstream signaling components. TGF-alpha and related peptides play an important role in the progression of cancer as well as in neuropathological processes (5,6).

1. Derynck, R. (1986) J. Cell Biochem. 32, 203-204.
2. de Larco, J.E. and Tordaro, G.J. (1978) Proc. Natl. Acad. Sci. USA 75, 4001-4005.
3. Roberts, A. B. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 5339-5343.
4. Wong, S. T. et al. (1989) Cell 56, 495-506.
5. Rusch, V. et al. (1996) Cytokine Growth Factor Rev. 7, 133-141.
6. Junier, M.P. (2000) Prog. Neurobiol. 62, 443-473.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.