ZO-3 (D57G7) XP? Rabbit mAb

• 产品编号：CST-3704P      品牌：Cell Signaling Technology       原厂货号：3704P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ZO-3 (D57G7) XP®兔源单抗检测内源性总的ZO-3蛋白。单抗由合成肽段免疫动物产生，该肽段对应ZO-3的C末端残基。紧密连接或闭锁小带，横跨上皮细胞核内皮细胞，形成一个连续障碍到液体。它们调控细胞旁通透性保持细胞极性，阻断跨膜蛋白在顶点和底外侧的细胞表面之运动（综述1），闭锁小带蛋白ZO-1, -2, 和 -3（也称TJP1, 2,和3）是周围衔接蛋白，此蛋白连接接合性跨膜蛋白（比如occludin 和claudin）到肌动蛋白细胞骨架（综述2）。ZO-1 和-2对于紧密连接的形成和起作用是必需的（3,4）。在未完全融汇的增殖细胞中，ZO-1和-2已经显示共定位于核，并在转录调控中起重要 作用，很可能通过帮助转录调控因子的核的输入/输出 来完成（5-7）。ZO-2基因被两个启动子转录，产生ZO-2A和ZO-2C两个异构体，ZO-2C缺乏一个23个氨基酸的氨基末端序列，但在另一异构体中被发现。但是两个异构体都广泛表达，ZO-2基因的不正常调控可能与导管癌发展正相关（8）。外源表达的ZO-3的氨基末端产生显性负效应，干扰紧密连接和粘附连接的组装（9）。但是，其他的证据显示在没有ZO-3时，紧密连接确实形成（10）。小鼠缺乏ZO-3发育正常（11）。

1. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
2. Matter, K. and Balda, M.S. (2007) J Cell Sci 120, 1505-11.
3. Hernandez, S. et al. (2007) Exp Cell Res 313, 1533-47.
4. Umeda, K. et al. (2006) Cell 126, 741-54.
5. Betanzos, A. et al. (2004) Exp Cell Res 292, 51-66.
6. Traweger, A. et al. (2003) J Biol Chem 278, 2692-700.
7. Huerta, M. et al. (2007) Mol Biol Cell 18, 4826-36.
8. Chlenski, A. et al. (2000) Biochim Biophys Acta 1493, 319-24.
9. Wittchen, E.S. et al. (2000) J Cell Biol 151, 825-36.
10. Adachi, M. et al. (2006) Mol Cell Biol 26, 9003-15.
11. Xu, J. et al. (2008) Mol Cell Biol 28, 1669-78.

原厂资料：

Specificity / Sensitivity

ZO-3 (D57G7) XP® Rabbit mAb detects endogenous levels of total ZO-3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human ZO-3.

Background

Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 2). ZO-1 and -2 are required for tight junction formation and function (3,4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7). The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer (8).

Exogenous expression of the amino terminal portion of ZO-3 exerts a dominant negative effect that interferes with assembly of tight junctions and adherens junctions (9). However, additional evidence indicates that tight junctions do form in the absence of ZO-3 protein (10), and that mice lacking ZO-3 appear to develop normally (11).

1. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
2. Matter, K. and Balda, M.S. (2007) J Cell Sci 120, 1505-11.
3. Hernandez, S. et al. (2007) Exp Cell Res 313, 1533-47.
4. Umeda, K. et al. (2006) Cell 126, 741-54.
5. Betanzos, A. et al. (2004) Exp Cell Res 292, 51-66.
6. Traweger, A. et al. (2003) J Biol Chem 278, 2692-700.
7. Huerta, M. et al. (2007) Mol Biol Cell 18, 4826-36.
8. Chlenski, A. et al. (2000) Biochim Biophys Acta 1493, 319-24.
9. Wittchen, E.S. et al. (2000) J Cell Biol 151, 825-36.
10. Adachi, M. et al. (2006) Mol Cell Biol 26, 9003-15.
11. Xu, J. et al. (2008) Mol Cell Biol 28, 1669-78.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.