GRB10 Antibody

• 产品编号：CST-3702S      品牌：CST       原厂货号：3702S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GRB10 Antibody兔多抗检测内源性总GRB10蛋白异形体1的水平。该抗体不与GRB7 、GRB14蛋白发生交叉反应。该多克隆抗体通过使用人源 GRB10蛋白胺端残基的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。GRB7家族的受体蛋白包括 GRB7, GRB10 和GRB14，它们都包含一个胺端脯氨酸富集的SH3结合结构域，并接有PH, PBS 和SH2 结构域。该家族的每个成员都有一些剪切异构体(1)。据报道GRB10蛋白与许多受体酪氨酸激酶(RTKs)以及下游信号分子如Raf, Akt and Nedd4，发生反应(1,2)。虽然最初人们认为GRB10蛋白只有丝氨酸残基被磷酸化(3)，但是Src激酶家族成员出现酪氨酸（67位点）磷酸化的GRB10蛋白 (4)。

1. Han, D. C. et al. (2001) Oncogene 20, 6315-6321.
2. Jahn, T. et al. (2002) Mol. Cell. Biol. 22, 979-991.
3. Ooi, J. et al. (1995) Oncogene 10, 1621-1630.
4. Langlais, P. et al. (2000) Oncogene 19, 2895-2903.

原厂资料：

Specificity / Sensitivity

GRB10 Antibody detects endogenous levels of isoform 1 of total GRB10 protein independent of phosphorylation. The antibody does not cross-react with GRB7 or GRB14.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human GRB10. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The GRB7 family of adaptor proteins consist of GRB7, GRB10 and GRB14, which all contain an amino-terminal proline-rich SH3 binding domain, followed by PH, PBS and SH2 domains. Each member of the family has several splice variants (1). It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules such as Raf, Akt and Nedd4 (1,2). Although it was originally thought that GRB10 is exclusively phosphorylated at serine residues (3), Src kinase family members have been shown to phosphorylate GRB10 at Tyr67 (4).

1. Han, D. C. et al. (2001) Oncogene 20, 6315-6321.
2. Jahn, T. et al. (2002) Mol. Cell. Biol. 22, 979-991.
3. Ooi, J. et al. (1995) Oncogene 10, 1621-1630.
4. Langlais, P. et al. (2000) Oncogene 19, 2895-2903.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.