Perforin Antibody (Mouse Specific)

• 产品编号：CST-3693S      品牌：CST       原厂货号：3693S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Perforin Antibody (Mouse Specific)能够检测内源的 Perforin 总蛋白。该多克隆抗体是采用合成的小鼠 perforin 蛋白亮氨酸(349位)周围残基相对应的肽段免疫动物而制备的。颗粒酶是一个丝氨酸蛋白酶家族，由细胞毒性T淋巴细胞和自然杀伤细胞（NK）表达，是病原体和转化细胞免疫反应的关键组分（1）。颗粒酶作为酶原被合成，通过裂解前导序列被加工成成熟的酶。穿孔素是一种膜孔隙形成蛋白，它们在含有穿孔素的溶酶体样颗粒中通过胞外分泌方式被释放。颗粒酶B在所有颗粒酶中具有最强的细胞凋亡能力，由于它类似caspase，能够在天冬氨酸残基切割底物，从而直接激活Caspase酶原，切开下游的半胱天冬酶底物（2,3）。穿孔素是一种孔隙形成的蛋白质，促进细胞毒素丝氨酸蛋白酶如颗粒酶，导入靶细胞（4）。穿孔素主要在细胞毒性T淋巴细胞和NK细胞中表达。

1. Trapani, J.A. (2001) Genome Biol. 2, REVIEWS 3014.
2. Lord, S.J. et al. (2003) Immunol. Rev. 193, 31-8.
3. Trapani, J.A. and Sutton, V.R. (2003) Curr. Opin. Immunol. 15, 533-43.
4. Catalfamo, M. and Henkart, P.A. (2003) Curr. Opin. Immunol. 15, 522-7.

原厂资料：

Specificity / Sensitivity

Perforin Antibody (Mouse Specific) detects endogenous levels of total Perforin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding leucine 349 of mouse perforin.

Background

Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3).

Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells (4). Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells.

1. Trapani, J.A. (2001) Genome Biol. 2, REVIEWS 3014.
2. Lord, S.J. et al. (2003) Immunol. Rev. 193, 31-8.
3. Trapani, J.A. and Sutton, V.R. (2003) Curr. Opin. Immunol. 15, 533-43.
4. Catalfamo, M. and Henkart, P.A. (2003) Curr. Opin. Immunol. 15, 522-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.