Phospho-eEF2k (Ser366) Antibody

• 产品编号：CST-3691P      品牌：Cell Signaling Technology       原厂货号：3691P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-eEF2k (Ser366) Antibody检测仅在Ser366位点磷酸化的内源性eEF2k蛋白水平。通过人工合成人源eEF2k蛋白Ser366位点周围序列相应的磷酸化片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Eukaryotic elongation factor 2 kinase (eEF2k)使eEF2蛋白磷酸化并失活，从而导致多肽链延伸过程受到抑制(1)。通常eEF2k是一种Ca2+离子和钙调蛋白依赖性激酶(2,3)。eEF2激酶也能被PKA所激活(7-9)，而PKA通常是响应因应激增加或饥饿相关条件下cAMP水平的上升(4-6)。eEF2k也能够在促细胞生长和蛋白质合成的众多刺激下被调节。这种调节分别涉及到通过p90RSK对eEF2K的磷酸化，p70 S6 kinase在Ser366位点或SAPK4/p38delta在Ser359位点对eEF2K的磷酸化，从而导致eEF2k失活(7,8)。而这有助于eEF2的去磷酸化，同时促进蛋白质翻译。

1. Ryazanov, A.G. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 4884-4889.
2. Nairn, A.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 7839-7943.
3. Palfrey, H.C. et al. (1987) J. Biol. Chem. 262, 9785-9792.
4. Redpath, N.T. et al. (1993) Biochem. J. 293, 31-34.
5. Diggle, T.A. et al. (1998) Biochem. J. 336, 525-529.
6. Hovland, R. et al. (1999) FEBS Lett. 444, 97-101.
7. Wang, X. et al. (2001) EMBO J. 20, 4370-4379.
8. Knebel, A. et al. (2001) EMBO J. 20, 4360-4369.

原厂资料：

Specificity / Sensitivity

Phospho-eEF2k (Ser366) Antibody detects endogenous levels of eEF2k only when phosphorylated at Ser366.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser366 of human eEF2k. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Eukaryotic elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation (1). eEF2k is normally dependent on Ca2+ ions and calmodulin (2,3). It can be activated by PKA in response to elevated cAMP levels (4-6), which are generally increased in stress- or starvation-related conditions. eEF2k can also be regulated in response to a wide range of stimuli that promote cell growth and protein synthesis. This involves the phosphorylation of eEF2k by p90RSK and p70 S6 kinase at Ser366 or by SAPK4/p38delta at Ser359, leading to the inactivation of eEF2k (7,8), which facilitates the dephosphorylation of eEF2, and thus promotes translation.

1. Ryazanov, A.G. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 4884-4889.
2. Nairn, A.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 7839-7943.
3. Palfrey, H.C. et al. (1987) J. Biol. Chem. 262, 9785-9792.
4. Redpath, N.T. et al. (1993) Biochem. J. 293, 31-34.
5. Diggle, T.A. et al. (1998) Biochem. J. 336, 525-529.
6. Hovland, R. et al. (1999) FEBS Lett. 444, 97-101.
7. Wang, X. et al. (2001) EMBO J. 20, 4370-4379.
8. Knebel, A. et al. (2001) EMBO J. 20, 4360-4369.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.