TRADD (7G8) Rabbit mAb

• 产品编号：CST-3684S      品牌：CST       原厂货号：3684S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TRADD (7G8) Rabbit mAb兔单抗能够检测内源TRADD总蛋白。该单克隆抗体是由合成的人源TRADD蛋白甘氨酸227周围残基相应的肽段免疫动物而制备的。FasL和TNF-α等死亡因子介导的细胞凋亡，参与死亡诱导信号复合物（DISC）到它们各自的受体的形成过程（1）。其受体的配体激活后，Fas和TNF-R1与死亡结构域（DD）相互作用，其中DD含有接头蛋白FADD（FAS相关死亡域）（2,3）和TRADD（TNF-R1相关死亡域）（4）。 除了其羧基末端DD，FADD含有一个氨基末端的死亡效应结构域（DED），结合到caspase-8导致胱天蛋白酶抑制子的活化（5,6）。随后，caspase-8激活下游的效应胱天蛋白酶，如caspase-3，导致细胞凋亡过程中蛋白的裂解。与FADD不同，TRADD不含有DED（4）。结合到TRADD的TNF-R1驱动细胞凋亡，并与TRADD及FADD发生相互作用，进而导致caspase-8的活化（7）。

1. Nagata, S. (1997) Cell 88, 355-65.
2. Chinnaiyan, A.M. et al. (1995) Cell 81, 505-12.
3. Boldin, M.P. et al. (1995) J. Biol. Chem. 270, 7795-8.
4. Hsu, H. et al. (1995) Cell 81, 495-504.
5. Muzio, M. et al. (1996) Cell 85, 817-27.
6. Boldin, M.P. et al. (1996) Cell 85, 803-15.
7. Hsu, H. et al. (1996) Cell 84, 299-308.

原厂资料：

Specificity / Sensitivity

TRADD (7G8) Rabbit mAb detects endogenous levels of total TRADD protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding glycine 227 of human TRADD.

Background

Apoptosis mediated by death factors like FasL and TNF-α involves the formation of a death-inducing signaling complex (DISC) to their respective receptors (1). Upon ligand activation to their receptors, Fas and TNF-R1 associate with death domain (DD) containing adaptor proteins FADD (Fas associated death domain) (2,3) and TRADD (TNF-R1 associated death domain) (4). In addition to its carboxy-terminal DD, FADD contains an amino-terminal death effector domain (DED) that binds to DEDs found on caspase-8 which leads to activation of this initiator caspase (5,6). Caspase-8 subsequently activates downstream effector caspases, like caspase-3, resulting in the cleavage of proteins involved in the execution of apoptosis. Unlike FADD, TRADD does not contain a DED (4). Apoptosis driven by TNF-R1 binding to TRADD involves association of TRADD and FADD which then leads to activation of caspase-8 (7).

1. Nagata, S. (1997) Cell 88, 355-65.
2. Chinnaiyan, A.M. et al. (1995) Cell 81, 505-12.
3. Boldin, M.P. et al. (1995) J. Biol. Chem. 270, 7795-8.
4. Hsu, H. et al. (1995) Cell 81, 495-504.
5. Muzio, M. et al. (1996) Cell 85, 817-27.
6. Boldin, M.P. et al. (1996) Cell 85, 803-15.
7. Hsu, H. et al. (1996) Cell 84, 299-308.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.