Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb

产品编号：CST-3677S 品牌：CST 原厂货号：3677S
产品分类：抗体 > 二抗 > 非结合unconjugated
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包装：

运保温度:

–20°C

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描述：

Mouse Anti-rabbit IgG (轻链特异性) (L57A3) mAb (HRP 标记)以天然的构象或变性还原形式识别兔源IgG的轻链，不能与兔源IgG变性和还原的重链(50KDa) 的结合。单克隆抗体是通过采用天然的全兔IgG免疫动物生产的。亲和纯化的mouse anti-rabbit IgG (Light-Chain Specific) antibody.该抗体已经过优化，可以作为二抗用于western blot实验。Mouse Anti-rabbit IgG (轻链特异性) (L57A3) mAb ) 仅与兔IgG的轻链发生反应，不能与变性还原的兔源IgG重链的结合。当免疫沉淀后进行western blotting时，一抗结合的变性的兔源IgG的重链的位置大约分别在50KDa，经常会有相同分子量的条带模糊的蛋白。采用 Mouse Anti-rabbit IgG (轻链特异性) (L57A3) mAb (HRP 标记)了一减少这种问题的发生。

原厂资料：

Specificity / Sensitivity

Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb recognizes the light chain of rabbit IgG, either in native conformation or denatured and reduced form. It does not bind to the denatured and reduced rabbit IgG heavy chain (about 50 kDa) on western blot.

Source / Purification

Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

Description

Affinity purified mouse anti-rabbit IgG (Light-Chain Specific) antibody.

Applications

This product has been optimized for use as a secondary antibody in Western blotting applications.

Background

Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb only reacts with the light chain of rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG heavy chain of the primary antibody used for IP runs at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have a similar molecular weight. Using Mouse Anti-Rabbit IgG (Light-Chain Specific) (L57A3) mAb as a secondary antibody will eliminate this problem.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.