Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody

• 产品编号：CST-3674S      品牌：CST       原厂货号：3674S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody仅检测threonine 18和serine 19位点都磷酸化的内源性myosin light chain 2 (平滑肌)蛋白。该抗体不能与myosin light chain 2的其它亚型发生交叉反应。通过人工合成人源myosin light chain 2蛋白Thr18/Ser19位点周围相应的磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Myosin蛋白是由六个多肽链组成：两个相同的重链和两对轻链。Myosin light chain 2 (MLC2)又称为myosin regulatory light chain (MRLC or RLC, LC20)，它有许多依赖它的分布的亚型。在平滑肌里，MLC2通过myosin light chain kinase (MLCK)以一个Ca2+-钙调蛋白依赖性方式使其在Thr18和Ser19 位点磷酸化(1)。这个磷酸化是与myosin ATPase活性和平滑肌收缩有关(2)。ROCK蛋白也使平滑肌MLC2蛋白Ser19位点磷酸化，这将调节应激纤维的装配(3)。通过PKC激酶使平滑肌MLC2蛋白Ser1/Ser2 and Ser9 位点磷酸化已经被报道抑制myosin ATPase活性 (4,5) 。通过cdc2激酶的磷酸化控制胞质分裂的时间(5)。在心肌亚型上缺乏磷酸化位点的转基因老鼠显示形态和功能的异常(6)。

1. Ikebe, M. and Hartshorne, D.J. (1985) J. Biol. Chem. 260, 10027-10031.
2. Tan, J. L. et al. (1992) Annu. Rev. Biochem. 61, 721-759.
3. Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806.
4. Ikebe, M. et al. (2000) J. Biol. Chem. 262, 9569-9573.
5. Satterwhite, L. L. et al. (1992) J. Cell Biol. 118, 595-605.
6. Sanbe, A. et al. (1999) J. Biol. Chem. 274, 21085-21094.

原厂资料：

Specificity / Sensitivity

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody detects endogenous levels of myosin light chain 2 (smooth muscle) only when dually phosphorylated at threonine 18 and serine 19. The antibody does not cross-react with the cardiac isoform of myosin light chain 2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr18/Ser19 of human myosin light chain 2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6).

1. Ikebe, M. and Hartshorne, D.J. (1985) J. Biol. Chem. 260, 10027-10031.
2. Tan, J. L. et al. (1992) Annu. Rev. Biochem. 61, 721-759.
3. Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806.
4. Ikebe, M. et al. (2000) J. Biol. Chem. 262, 9569-9573.
5. Satterwhite, L. L. et al. (1992) J. Cell Biol. 118, 595-605.
6. Sanbe, A. et al. (1999) J. Biol. Chem. 274, 21085-21094.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.