cdc25A Antibody

• 产品编号：CST-3652S      品牌：CST       原厂货号：3652S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

 cdc25A Antibody 能够识别内源性cdc25A总蛋白。该抗体在被检测组织富含小牛肠磷酸酶时会与其发生交叉反应。该多克隆抗体是由合成的人源的针对cdc25A蛋白丝氨酸177位点处肽段免疫动物而生产的。 cdc25蛋白磷酸酶家族在激活周期蛋白依赖性激酶(CDKs)的过程中发挥关键作用，这种作用主要通过去磷酸化保守的苏氨酸14/15位点。cdc25C是激活CDK1从而克服G2/M期检验点促进有丝分裂的首要原因。cdc25A的主要底物是CDK2，激活状态的CDK2允许G1/S期和S期检验点的进展(1)。各种机制如磷酸化、泛素化和14-3-3蛋白结合抑制等紧密控制cdc25A的丰度、亚细胞定位和活性。在正常的细胞周期进程中，转录因子c-Myc和E2F水平的升高可以导致cdc25A表达的增加(2)。当条件有利于DNA合成时，cdc25A和CDK2组成一个激活回路，其中分别激活其它的酶类(1)。另一方面，DNA损伤可以通过Chk1和Chk2引起多个抑制性位点(Ser123, Ser177, Ser278, Ser292, and Thr506)的磷酸化，最终导致14-3-3蛋白结合和泛素介导的降解(3,4)。

1. Hoffmann, I. et al. (1994) EMBO J. 13, 4302-4310.
2. Vigo, E. et al. (1999) Mol. Cell. Biol. 19, 6379-6395.
3. Chen, M. et al. (2003) Mol. Cell. Biol. 23, 7488-7497.
4. Sorensen, C.S. et al. (2003) Cancer Cell 3, 247-258.

原厂资料：

Specificity / Sensitivity

cdc25A Antibody recognizes endogenous levels of total cdc25A protein. The antibody will cross-react with calf intestinal phosphatase (CIP) when present in abundance.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser177 of human cdc25A.

Background

The cdc25 protein phosphatase family plays a critical role in activating cyclin-dependent kinases (CDKs) via dephosphorylation of conserved Thr14/Tyr15 inhibitory phosphorylation sites. While cdc25C is primarily responsible for activating CDK1 to overcome the G2/M checkpoint and allow mitotic entry, the primary substrate of cdc25A is CDK2, which, when active, allows progression through the G1/S and intra-S checkpoints (1). Abundance, subcellular localization and activity of cdc25A is tightly controlled by a variety of mechanisms, including phosphorylation, ubiquitination, and inhibitory binding to 14-3-3 proteins. During normal cell cycle progression, elevated c-Myc and E2F transcription factor levels lead to increased cdc25A expression (2). When conditions are favorable for DNA synthesis, cdc25A and CDK2 form an activation loop, wherein each activates the other enzyme (1). DNA damage, on the other hand, leads to multisite phosphorylation at inhibitory sites (Ser123, Ser177, Ser278, Ser292, and Thr506) by Chk1 and Chk2, which result in 14-3-3 binding and ubiquitin-mediated degradation (3,4).

1. Hoffmann, I. et al. (1994) EMBO J. 13, 4302-4310.
2. Vigo, E. et al. (1999) Mol. Cell. Biol. 19, 6379-6395.
3. Chen, M. et al. (2003) Mol. Cell. Biol. 23, 7488-7497.
4. Sorensen, C.S. et al. (2003) Cancer Cell 3, 247-258.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.