PRC1 Antibody

• 产品编号：CST-3639S      品牌：CST       原厂货号：3639S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PRC1抗体可以识别内源性总的PRC1蛋白。多抗由合成肽段免疫动物产生，该肽段与人PRC1蛋白522位甘氨酸附近氨基酸一致。抗体由蛋白A和肽段亲和层析技术纯化得到。胞质分裂调控蛋白1 (PRC1)是非肌动蛋白微管偶联蛋白MAP65/ASE1家族的一员，最初在出芽酵母中被发现（1,2）。PRC1是CKD1的底物，CDK1能够保持PRC1在失活的，单体的状态（2）。CDK1的细胞周期依赖性降解会导致PRC1的去磷酸化随后被KIF4介导转位到微管的两端，这将促进交联反向平行微管使微管集合成束（3-5）。PRC1集合微管成束的功能在维持胞质分裂过程中纺锤体中央区的结构完整性至关重要（3,6,7）。

1. Pellman, D. et al. (1995) J Cell Biol 130, 1373-85.
2. Jiang, W. et al. (1998) Mol Cell 2, 877-85.
3. Kurasawa, Y. et al. (2004) EMBO J 23, 3237-48.
4. Zhu, C. and Jiang, W. (2005) Proc Natl Acad Sci USA 102, 343-8.
5. Subramanian, R. et al. (2010) Cell 142, 433-43.
6. Mollinari, C. et al. (2002) J Cell Biol 157, 1175-86.
7. Zhu, C. et al. (2006) Proc Natl Acad Sci USA 103, 6196-201.

原厂资料：

Specificity / Sensitivity

PRC1 Antibody recognizes endogenous levels of total PRC1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly522 of human PRC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Protein Regulator of Cytokinesis 1 (PRC1) is a member of the MAP65/ASE1 family of nonmotor microtubule-associated proteins, first described in budding yeast (1,2). PRC1 is a substrate of CDK1, which maintains PRC1 in an inactive, monomeric state (2). Cell-cycle dependent degradation of CDK1 leads to dephosphorylation of PRC1 and subsequent KIF4-mediated translocation to the plus ends of microtubules, where it promotes microtubule bundling by cross-linking antiparallel microtubules (3-5). The microtubule bundling functions of PRC1 play a critical role in maintaining structural integritry of the spindle midzone during cytokinesis (3,6,7).

1. Pellman, D. et al. (1995) J Cell Biol 130, 1373-85.
2. Jiang, W. et al. (1998) Mol Cell 2, 877-85.
3. Kurasawa, Y. et al. (2004) EMBO J 23, 3237-48.
4. Zhu, C. and Jiang, W. (2005) Proc Natl Acad Sci USA 102, 343-8.
5. Subramanian, R. et al. (2010) Cell 142, 433-43.
6. Mollinari, C. et al. (2002) J Cell Biol 157, 1175-86.
7. Zhu, C. et al. (2006) Proc Natl Acad Sci USA 103, 6196-201.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.