Nicastrin Antibody

• 产品编号：CST-3632S      品牌：CST       原厂货号：3632S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Nicastrin Antibody 兔多抗识别内源性的Nicastrin 总蛋白。该多克隆抗体由合成的人类Nicastrin蛋白羧基末端附近肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。Nicastrin是一种跨膜糖蛋白，是γ-分泌酶复合物的重要组成部分（1,2）。Nicastrin物理上与presenilin结合，在presenilin的稳定和正确的定位到膜结合的γ-分泌酶复合物上起着重要作用（3）。Nicastrin也作为γ-分泌酶底物如APP和Notch的接合位点，直接与它们结合，将它们提交给γ-分泌酶以确保正确裂解过程（2,4）。

1. Yu, G. et al. (2000) Nature 407, 48-54.
2. Esler, W. P. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 2720-2725.
3. Kopan, R. and Goate, A. (2002) Neuron 33, 321-324.
4. Chen, F. et al. (2001) Nat. Cell Biol. 3, 751-754.

原厂资料：

Specificity / Sensitivity

Nicastrin Antibody detects endogenous levels of Nicastrin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human Nicastrin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (1,2). Nicastrin is physically associated with presenilin and plays an important role in the stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (3). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and properly presenting them to γ-secretase to ensure the correct cleavage process (2,4).

1. Yu, G. et al. (2000) Nature 407, 48-54.
2. Esler, W. P. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 2720-2725.
3. Kopan, R. and Goate, A. (2002) Neuron 33, 321-324.
4. Chen, F. et al. (2001) Nat. Cell Biol. 3, 751-754.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.