NUT (C52B1) Rabbit mAb

• 产品编号：CST-3625S      品牌：CST       原厂货号：3625S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NUT (C52B1) Rabbit mAb兔单抗能够检测内源性NUT总蛋白水平。该抗体在NUT midline carcinoma (NMC)中也能检测内源性BRD4-NUT融合蛋白。通过重组人源NUT蛋白去免疫动物从而制备出此单克隆抗体。Nuclear protein in testis (NUT)通常局限于睾丸和卵巢的生殖细胞中(1,2)。NUT midline carcinoma (NMC)是一个最近公认的癌症，它通过染色质重排的存在而定义的，这涉及染色体15q14 上的NUT基因(3)。大部分情况下，染色体易位发生在染色体19的NUT和BRD4之间，这导致BRD4-NUT 融合蛋白的形成。在剩余的肿瘤中，不同的NUT重排存在涉及BRD3，它是一个BRD4的紧密的同系物。BRD4-NUT和BRD3-NUT编码融合蛋白，这些蛋白出现通过封闭上皮细胞分化从而有助于致癌。NMCs是侵入性和高度致死癌症，它可以从形态上区分其它弱分化的癌症。假如内源性NUT蛋白限制性表达，则该抗体能通过免疫组化和免疫荧光分析NUT融合蛋白(2)。

1. French, C.A. et al. (2003) Cancer Res 63, 304-7.
2. Haack, H. et al. (2009) Am J Surg Pathol 33, 984-91.
3. French, C.A. et al. (2008) Oncogene 27, 2237-42.

原厂资料：

Specificity / Sensitivity

NUT (C52B1) Rabbit mAb detects endogenous levels of total NUT protein. The antibody also detects endogenous levels of the BRD4-NUT fusion protein found in NUT midline carcinoma (NMC).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to the human NUT protein.

Background

Nuclear protein in testis (NUT) is normally confined to the germ cells of the testis and ovary (1,2). NUT midline carcinoma (NMC) is a recently recognized cancer that is defined by the presence of chromosomal rearrangements involving the NUT gene on chromosome 15q14 (3). In most cases the chromosomal translocation occurs between NUT and BRD4 on chromosome 19, resulting in the formation of a BRD4-NUT fusion protein. In the remaining tumors, variant NUT rearrangements are present involving BRD3, a very close homolog of BRD4. BRD4-NUT and BRD3-NUT encode fusion proteins that appear to contribute to carcinogenesis by blocking epithelial cell differentiation. NMCs, which are aggressive and highly lethal carcinomas, are morphologically indistinguishable from other poorly differentiated carcinomas. Given the limited expression of endogenous NUT protein, this antibody can be used to detect NUT fusion proteins in tissues by immunohistochemistry and immunofluorescence (2).

1. French, C.A. et al. (2003) Cancer Res 63, 304-7.
2. Haack, H. et al. (2009) Am J Surg Pathol 33, 984-91.
3. French, C.A. et al. (2008) Oncogene 27, 2237-42.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.