β-Tubulin (9F3) Rabbit mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-3623S      品牌：CST       原厂货号：3623S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 488 Conjugate)检测内源性β-tubulin总蛋白，但是不能与重组的α-tubulin蛋白发生交叉反应。通过人工合成人源β-tubulin蛋白氨基端相应的磷酸化片段去免疫动物从而制备出单克隆抗体。Cell Signaling Technology抗体连接着Alexa Fluor® 488 fluorescent dye，并且在人类细胞中直接用流式细胞仪和免疫荧光分析方法进行内部检测。该抗体被期望与非连接的β-Tubulin (9F3) Rabbit mAb #2128有相同的物种交叉反应。细胞骨架由三种类型的细胞质基质纤维组成：微管、微丝(肌动蛋白纤维)和中间纤维。球形微管蛋白亚基包含微管建筑块，由α/β-tubulin异源二聚体形成所有真核细胞的tubulin亚单位。γ-tubulin被需要在tubulin亚单位的成核聚合作用中去形成微管聚合物。许多细胞运动是通过微管动力介导的，包括纤毛和鞭毛的跳动、膜泡的核质转运、在减数分裂/有丝分裂期间染色体排布和神经轴突的迁移。这些运动由于竞争性微管聚合作用和解聚作用或通过微管动力蛋白的运动(1)。

1、Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

原厂资料：

Specificity / Sensitivity

β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total β-tubulin protein and does not cross-react with recombinant α-tubulin.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the amino terminus of human β-tubulin.

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

1、Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.