Phospho-Tuberin/TSC2 (Ser1254) Antibody

• 产品编号：CST-3616S      品牌：CST       原厂货号：3616S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

只有在Ser1254位点磷酸化后，Phospho-Tuberin/TSC2 (Ser1254)抗体检测内源性的tuberin（马铃薯球蛋白）的含量。 该抗体不能检测tuberin蛋白的其他磷酸化位点。多克隆抗体是采用与人类tuberin（马铃薯球蛋白）Ser1254残基周围相对应的磷酸盐肽免疫动物生产的。多克隆抗体采用蛋白A和肽亲和色谱法纯化。铃薯球蛋白（Tuberin）是TSC2肿瘤抑制基因的产物，是细胞增殖和肿瘤发展的重要调节因子（1）。TSC2或与之相关的TSC1（hamartin）的突变可以引起结节硬化复合体TSC（tuberous sclerosis complex），是一种常染色体显性遗传病，其特点为发生多种广泛的非恶性肿瘤（2）。Tuberin可以在Thr1462位点直接被Akt/PKB 磷酸化（3）。 在Thr1462 和 Tyr1571位点的磷酸化可以调节tuberin-hamartin复合体和tuberin的活性（3-5）。另外，tuberin可以抑制mTOR（mammalian target of rapamycin）， 进而推动p70 S6激酶的抑制和真核细胞抑制因子4E结合蛋白（4E-BP1，一种转录起始抑制因子）的活性，并最终抑制翻译（3,6,7）。

P38活化激酶MK2 (MAPKAPK-2) 能够磷酸化马铃薯球蛋白（tuberin）的Ser1254 位点，增强tuberin 和14-3-3 间的作用（8）。

1. Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658.
2. Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119.
3. Manning, B. D. et al. (2002) Mol. Cell 10, 151-161.
4. Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.
5. Dan, H. C. et al. (2002) J. Biol. Chem. 277, 35364-35370.
6. Goncharova, E.A. et al. (2002) J. Biol. Chem. 277, 30958-30967.
7. Inoki, K. et al. (2002) Nat. Cell Biol. 4, 648-657.
8. Li, Y. et al. (2003) J. Biol. Chem. 278, 13663-13671.

原厂资料：

Specificity / Sensitivity

Phospho-Tuberin/TSC2 (Ser1254) Antibody detects endogenous levels of tuberin only when phosphorylated at serine 1254. This antibody does not detect tuberin phosphorylated at other sites.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1254 of human tuberin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).p38-activated kinase MK2 (MAPKAPK-2) phosphorylates Ser1254 of tuberin, and thus augments the interaction between tuberin and 14-3-3 (8).

1. Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658.
2. Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119.
3. Manning, B. D. et al. (2002) Mol. Cell 10, 151-161.
4. Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.
5. Dan, H. C. et al. (2002) J. Biol. Chem. 277, 35364-35370.
6. Goncharova, E.A. et al. (2002) J. Biol. Chem. 277, 30958-30967.
7. Inoki, K. et al. (2002) Nat. Cell Biol. 4, 648-657.
8. Li, Y. et al. (2003) J. Biol. Chem. 278, 13663-13671.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.