NCAM (CD56) Antibody

• 产品编号：CST-3606S      品牌：CST       原厂货号：3606S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NCAM (CD56)抗体测内源性总NCAM 的水平。多克隆抗体由合成肽段免疫动物产生，该肽段与人NCAM的800位氨基酸附近残基对应。抗体由蛋白A和肽段亲和层析纯化得到。NCAM(神经细胞粘附分子, CD56)是一个粘附糖蛋白，带有五个胞外免疫球蛋白样结构域，跟着两个纤连蛋白III型重复单元。结构多样性是通过选择性剪接导致不同的胞质结构域造成（1）。NCAM通过homo和异染性的相互作用调节神经附着，轴突延伸和细胞间相互作用。PSA(多唾液酸)翻译后修饰NCAM并增加小细胞肺癌转移潜能，Wilms+肿瘤，神经细胞瘤和横纹肌肉瘤（2）。CD56和CD16普遍用于确定NK细胞了，虽然一些带有T细胞标志物CD3和CD4的细胞液表达CD56（3）。

1. Cunningham, B. A. et al. (1987) Science 236, 799-806.
2. Seidenfaden, R. et al. (2003) Mol. Cell. Biol. 23, 5908-5918.
3. Robertson, M.J. and Ritz, J. (1990) Blood 76, 2421-2438.

原厂资料：

Specificity / Sensitivity

NCAM (CD56) Antibody detects endogenous levels of total NCAM protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 800 of human NCAM. Antibodies are purified by protein A and peptide affinity chromatography.

Background

NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein with five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. Structural diversity is introduced by alternative splicing resulting in different cytoplasmic domains (1). NCAM mediates neuronal attachment, neurite extension and cell-cell interactions through homo and heterophilic interactions. PSA (polysialic acid) post-translationally modifies NCAM and increases the metastatic potential of small cell lung carcinoma, Wilms+ tumor, neuroblastoma and rhabdomyosarcoma (2). CD56 and CD16 are commonly used to identify NK cells although some cells with the T cell markers CD3 and CD4 also express CD56 (3).

1. Cunningham, B. A. et al. (1987) Science 236, 799-806.
2. Seidenfaden, R. et al. (2003) Mol. Cell. Biol. 23, 5908-5918.
3. Robertson, M.J. and Ritz, J. (1990) Blood 76, 2421-2438.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.